Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Immunocytochemistry and Immunohistochemistry

Immunocytochemistry and Immunohistochemistry

Immunocytochemistry (ICC) and immunohistochemistry (IHC) are techniques that use antibodies to check for specific proteins or antigens in a sample. The technique was first published by Albert Coons in 1941 to detect the presence of pneumococcal antigen in tissue sections from mice infected with Pneumococcus. Immunocytochemistry helps localization of proteins or antigens in individual cells like blood cells, stem cells, etc., while immunohistochemistry does the same for tissue samples.
These...

Immunogold Electron Microscopy

Immunogold Electron Microscopy

Immunoelectron microscopy utilizes immunogold labeling of endogenous proteins with specific antibodies to detect and localize these proteins in cells and tissues. The procedure provides insights into the distribution and quantification of protein under different stimulation conditions offering clues about their functions. Conjugating highly electron-dense gold particles with primary or secondary antibodies allow antigen detection on and within cells, with high resolution and specificity.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Activation of Akt signaling in prostate induces a TGFβ-mediated restraint on cancer progression and metastasis.

Oncogene·2013

Same author

Phase IB study of the combination of docetaxel, gemcitabine, and bevacizumab in patients with advanced or recurrent soft tissue sarcoma: the Axtell regimen.

Annals of oncology : official journal of the European Society for Medical Oncology·2011

Same author

IgA nephropathy in the triethnic population of New Mexico.

Clinical nephrology·2009

Same author

The role of SPARC in the TRAMP model of prostate carcinogenesis and progression.

Oncogene·2009

Same author

Ubiquitin E3 ligase WWP1 as an oncogenic factor in human prostate cancer.

Oncogene·2006

Same author

Neuromedin U is regulated by the metastasis suppressor RhoGDI2 and is a novel promoter of tumor formation, lung metastasis and cancer cachexia.

Oncogene·2006

Same journal

Erratum to: Immunotherapeutic Approach to Cancer with Cutaneous DNA Vaccination.

Methods in molecular medicine·2015

Same journal

Methods for cancer gene therapy using tumor suppressor genes.

Methods in molecular medicine·2014

Same journal

Suppression of the human carcinoma phenotype by an antioncogene ribozyme.

Methods in molecular medicine·2014

Same journal

Methods for the use of stromal cells for therapeutic gene therapy.

Methods in molecular medicine·2014

Same journal

Methods for adenovirus-mediated gene transfer to synovium in vivo.

Methods in molecular medicine·2014

Same journal

Methods for gene transfer to synovium.

Methods in molecular medicine·2014

See all related articles

Search research articles

Related Experiment Video

Updated: Jun 4, 2026

Immunohistochemical Analysis in the Rat Central Nervous System and Peripheral Lymph Node Tissue Sections

Immunohistochemical Analysis in the Rat Central Nervous System and Peripheral Lymph Node Tissue Sections

Published on: November 14, 2016

The immunohistochemical method.

L A Cerilli¹, H F Frierson

¹Department of Pathology, University of Virginia Health System, Charlottesville, VA.

Methods in Molecular Medicine

|February 15, 2011

Summary

This summary is machine-generated.

Immunohistochemistry uses antibodies to detect specific cellular antigens, aiding in cancer diagnosis and targeted therapy. This technique is valuable for analyzing protein expression in fixed tissues, facilitating large-scale cancer research.

More Related Videos

Histological-Based Stainings Using Free-Floating Tissue Sections

Histological-Based Stainings Using Free-Floating Tissue Sections

Published on: August 25, 2020

Immunohistochemistry Techniques to Analyze Cellular Proliferation and Neurogenesis in Rats Using the Thymidine Analog BrdU

Immunohistochemistry Techniques to Analyze Cellular Proliferation and Neurogenesis in Rats Using the Thymidine Analog BrdU

Published on: September 9, 2020

Related Experiment Videos

Last Updated: Jun 4, 2026

Immunohistochemical Analysis in the Rat Central Nervous System and Peripheral Lymph Node Tissue Sections

Immunohistochemical Analysis in the Rat Central Nervous System and Peripheral Lymph Node Tissue Sections

Published on: November 14, 2016

Histological-Based Stainings Using Free-Floating Tissue Sections

Histological-Based Stainings Using Free-Floating Tissue Sections

Published on: August 25, 2020

Immunohistochemistry Techniques to Analyze Cellular Proliferation and Neurogenesis in Rats Using the Thymidine Analog BrdU

Immunohistochemistry Techniques to Analyze Cellular Proliferation and Neurogenesis in Rats Using the Thymidine Analog BrdU

Published on: September 9, 2020

Area of Science:

Biomedical Sciences
Molecular Biology
Pathology

Background:

Immunohistochemistry (IHC) is a technique that utilizes antibody-antigen binding for visualization.
The increasing availability of antibodies enhances IHC's utility in research and diagnostics.
IHC is crucial for identifying specific cellular targets in various diseases.

Purpose of the Study:

To highlight the diagnostic and therapeutic applications of immunohistochemistry.
To emphasize the role of IHC in guiding targeted cancer therapies.
To underscore the utility of IHC in retrospective studies using fixed paraffin-embedded tissues.

Main Methods:

Antibody binding to cellular or tissue antigens.
Visualization of antibody-antigen complexes via a detection system.
Application in fixed paraffin-embedded tissues for protein expression analysis.

Main Results:

Immunohistochemistry serves as a valuable diagnostic tool.
It guides specific therapies targeting neoplastic cell antigens.
Enables large-scale retrospective studies of protein expression in human cancers.

Conclusions:

Immunohistochemistry is an indispensable technique in modern pathology and oncology.
Its application in fixed tissues facilitates extensive research into cancer protein expression.
IHC plays a key role in personalized medicine and cancer treatment strategies.