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Respiratory Syncytial Virus Disease01:29

Respiratory Syncytial Virus Disease

Human respiratory syncytial virus (RSV) is a widespread pathogen that primarily targets infants and young children but also poses a serious health risk to elderly and immunocompromised individuals. Belonging to the Pneumoviridae family, RSV is a negative-sense, single-stranded RNA virus within the Pneumovirus genus. Its global health burden is significant, with millions of cases annually resulting in hospitalizations and mortality, particularly in resource-limited settings. Although most...

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Updated: Jun 4, 2026

Assays for the Specific Growth Rate and Cell-binding Ability of Rotavirus
10:49

Assays for the Specific Growth Rate and Cell-binding Ability of Rotavirus

Published on: January 28, 2019

Rotavirus entry into tissue culture cells.

J M Gilbert1, H B Greenberg

  • 1Stanford University School of Medicine, Stanford, CA.

Methods in Molecular Medicine
|February 15, 2011
PubMed
Summary
This summary is machine-generated.

Rotavirus outer-layer proteins VP4 and VP7 are crucial for infection. VP4, especially after cleavage into VP8* and VP5*, plays a key role in rotavirus attachment and cell entry.

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Simplified Reverse Genetics Method to Recover Recombinant Rotaviruses Expressing Reporter Proteins
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Area of Science:

  • Virology
  • Molecular Biology
  • Infectious Diseases

Background:

  • Rotavirus (RV) is an icosahedral virus with a triple-protein layer.
  • Viral Protein 7 (VP7) is the major glycoprotein component of the outer layer.
  • Viral Protein 4 (VP4) forms dimers on the viral surface and is involved in cell attachment and entry.

Purpose of the Study:

  • To clarify the role of VP7 in viral entry.
  • To investigate the function of VP4 in rotavirus infectivity and cell entry.
  • To understand the significance of VP4 proteolytic cleavage into VP8* and VP5*.

Main Methods:

  • Analysis of viral protein functions in rotavirus infectivity.
  • Investigating the role of VP4 and VP7 in viral entry mechanisms.
  • Studying the impact of VP4 cleavage on viral infectivity.

Main Results:

  • VP4 is directly involved in rotavirus cell attachment and entry.
  • Proteolytic cleavage of VP4 into VP8* and VP5* significantly enhances viral infectivity.
  • VP4's role in host range and virulence is established.

Conclusions:

  • VP4 is a critical determinant of rotavirus cell entry and infectivity.
  • Cleavage of VP4 into VP8* and VP5* is essential for efficient viral infection.
  • Further research is needed to fully elucidate VP7's role in viral entry.