Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Video

Updated: Jun 4, 2026

Exploring the Arginine Methylome by Nuclear Magnetic Resonance Spectroscopy

Exploring the Arginine Methylome by Nuclear Magnetic Resonance Spectroscopy

Published on: December 16, 2021

Methylated polyamines as research tools.

Alex R Khomutov¹, Janne Weisell, Maxim A Khomutov

¹Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow 119991, Russia. alexkhom@list.ru

Methods in Molecular Biology (Clifton, N.J.)

|February 15, 2011

Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Synthesis and Biological Evaluation of New Quinazolin-4(3H)-One-Coumarin Hybrids Designed as Anticancer and Antibacterial Agents.

International journal of molecular sciences·2026

Same author

<i>H-</i>phosphinic analogs of natural amino acids: a novel and efficient treatment for preventing biodeterioration of treasured painted artworks.

Frontiers in microbiology·2026

Same author

IFNγ alters the aberrant phenotype of α-synuclein-treated microglia reducing the detrimental impact of their secretome on dopaminergic neurons.

Neuroscience·2026

Same author

5-Substituted 4-Thiouridines, 4-Thio-2'-deoxyuridines and Their Oligoglycol Carbonate Prodrugs as Promising Antimicrobial Agents.

International journal of molecular sciences·2025

Same author

A new urinary biomarker reflecting the progesterone excretion pattern identified through serum steroid and urinary progesterone metabolite analysis during pro-oestrus and oestrus in female dogs (Canis familiaris).

Animal reproduction science·2025

Same author

Serum and hair steroid profiles and their associations with body composition in adolescence.

European journal of endocrinology·2025

Researchers synthesized novel methylspermidines, including beta-methylspermidine (β-MeSpd) and gamma-methylspermidine (γ-MeSpd). These compounds, along with alpha-methylspermidine (α-MeSpd), are valuable for polyamine research in enzymology and cell biology.

Area of Science:

Organic Chemistry
Biochemistry
Molecular Biology

Background:

Polyamines are essential biological molecules involved in numerous cellular processes.
Specific polyamine analogs can serve as valuable tools for investigating biological functions.
Previous research has focused on certain polyamine structures, leaving gaps in the availability of others.

Purpose of the Study:

To synthesize previously unknown methyl-substituted polyamines: β-methylspermidine (β-MeSpd) and γ-methylspermidine (γ-MeSpd).
To develop synthetic routes for α-methylspermidine (α-MeSpd).
To provide novel tools for polyamine research.

Main Methods:

Synthesis of racemic β-MeSpd and γ-MeSpd via nitrile reduction and deprotection.
Preparation of α-MeSpd through alkylation of putrescine using a functionalized amino alcohol derivative.

More Related Videos

In vitro Methylation Assay to Study Protein Arginine Methylation

In vitro Methylation Assay to Study Protein Arginine Methylation

Published on: October 5, 2014

Quantitative Methods to Study Protein Arginine Methyltransferase 1-9 Activity in Cells

Quantitative Methods to Study Protein Arginine Methyltransferase 1-9 Activity in Cells

Published on: August 7, 2021

Related Experiment Videos

Last Updated: Jun 4, 2026

Exploring the Arginine Methylome by Nuclear Magnetic Resonance Spectroscopy

Exploring the Arginine Methylome by Nuclear Magnetic Resonance Spectroscopy

Published on: December 16, 2021

In vitro Methylation Assay to Study Protein Arginine Methylation

In vitro Methylation Assay to Study Protein Arginine Methylation

Published on: October 5, 2014

Quantitative Methods to Study Protein Arginine Methyltransferase 1-9 Activity in Cells

Quantitative Methods to Study Protein Arginine Methyltransferase 1-9 Activity in Cells

Published on: August 7, 2021

Utilized protecting group strategies (Boc, Cbz) and reduction/deprotection steps.

Main Results:

Successfully synthesized novel racemic β-MeSpd and γ-MeSpd.
Developed a method for synthesizing α-MeSpd.
The synthesized methylspermidines are chemically characterized and ready for biological application.

Conclusions:

Established synthetic pathways for key methylspermidine analogs.
The novel β-MeSpd, γ-MeSpd, and α-MeSpd are valuable chemical probes.
These compounds will advance studies in polyamine enzymology and cell biology.