Jove
Visualize
Contact Us

Related Concept Videos

DNA Microarrays02:34

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Reversal of the Leloir pathway to promote galactose and tagatose synthesis from glucose.

Cell reports. Physical science·2026
Same author

A Novel Phosphatase Reverses the Leloir Pathway to Promote Tagatose Synthesis from Glucose.

bioRxiv : the preprint server for biology·2025
Same author

Computational Investigation of Cytochrome P450-Catalyzed Oxetane Formation in Taxol Biosynthesis.

Biochemistry·2025
Same author

Enabling commercial success of industrial biotechnology.

Science (New York, N.Y.)·2021
Same author

Publisher Correction: Engineered bidirectional promoters enable rapid multi-gene co-expression optimization.

Nature communications·2021
Same author

Chemically Inducible Chromosomal Evolution (CIChE) for Multicopy Metabolic Pathway Engineering.

Methods in molecular biology (Clifton, N.J.)·2019
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jun 4, 2026

Kinase Inhibitor Screening In Self-assembled Human Protein Microarrays
13:22

Kinase Inhibitor Screening In Self-assembled Human Protein Microarrays

Published on: October 23, 2019

DNA-directed assembly microarray for protein and small molecule inhibitor screening.

Ng Jin Kiat1, Fritz Simeon, Too Heng Phon

  • 1Singapore-Massachusetts Institute of Technology Alliance (SMA), National University of Singapore, Singapore, Singapore.

Methods in Molecular Biology (Clifton, N.J.)
|February 15, 2011
PubMed
Summary

We developed a novel DNA-directed assembly microarray platform for high-throughput drug discovery. This method enhances target molecule identification and validation through solution-phase interactions and DNA probe assembly.

More Related Videos

A Guided Materials Screening Approach for Developing Quantitative Sol-gel Derived Protein Microarrays
10:44

A Guided Materials Screening Approach for Developing Quantitative Sol-gel Derived Protein Microarrays

Published on: August 26, 2013

Extracellular Protein Microarray Technology for High Throughput Detection of Low Affinity Receptor-Ligand Interactions
06:01

Extracellular Protein Microarray Technology for High Throughput Detection of Low Affinity Receptor-Ligand Interactions

Published on: January 7, 2019

Related Experiment Videos

Last Updated: Jun 4, 2026

Kinase Inhibitor Screening In Self-assembled Human Protein Microarrays
13:22

Kinase Inhibitor Screening In Self-assembled Human Protein Microarrays

Published on: October 23, 2019

A Guided Materials Screening Approach for Developing Quantitative Sol-gel Derived Protein Microarrays
10:44

A Guided Materials Screening Approach for Developing Quantitative Sol-gel Derived Protein Microarrays

Published on: August 26, 2013

Extracellular Protein Microarray Technology for High Throughput Detection of Low Affinity Receptor-Ligand Interactions
06:01

Extracellular Protein Microarray Technology for High Throughput Detection of Low Affinity Receptor-Ligand Interactions

Published on: January 7, 2019

Area of Science:

  • Biotechnology
  • Molecular Biology
  • Drug Discovery

Background:

  • Microarray platforms are crucial for drug discovery and analysis, particularly for small molecules and proteins.
  • Developing high-throughput, high-fidelity screening platforms is essential for efficient drug development.
  • Conventional microarray platforms have limitations in mimicking biological environments for probe-analyte interactions.

Purpose of the Study:

  • To present a novel DNA-directed assembly microarray platform.
  • To detail experimental protocols for implementing this platform in various applications.
  • To highlight improved screening and immobilization strategies for enhanced drug discovery.

Main Methods:

  • Utilizing a DNA-directed assembly technique on a dendrimer-modified glass surface.
  • Enabling solution-phase interactions between probes and analytes in a biological environment.
  • Describing protocols for antibody microarrays, RNA polymerase-DNA binding microarrays, and drug-screening microarrays.

Main Results:

  • The platform facilitates high-fidelity screening of potential target molecules.
  • It allows for solution-phase interactions, mimicking biological conditions more closely.
  • Demonstrated applicability across antibody, protein-DNA binding, and drug screening assays.

Conclusions:

  • The DNA-directed assembly microarray platform offers a robust and versatile tool for drug discovery.
  • This technique improves the efficiency and accuracy of identifying and validating drug targets.
  • The described protocols provide a foundation for broader adoption in molecular screening applications.