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Published on: May 21, 2017
Liposome-encapsulated hemoglobin accelerates gastric wound healing in the rat
Yuichi Okamoto1, Akira Kawaguchi, Yoshifumi Kise
1Department of Gastroenterological Surgery, Tokai University School of Medicine, Isehara, Kanagawa, Japan.
Background:
Liposome-encapsulated hemoglobin, a nanometer-sized artificial O2 carrier with a high O2 affinity (h-LEH), may facilitate O2 delivery to surgical wounds and thereby accelerate healing after gastrointestinal surgery.
Methods:
Ten mL/kg of h-LEH (n = 25), empty liposome (n = 21) or homologous washed red blood cells (RBC, n = 22) was intravenously infused prior to the creation of a 10 mm incision and interrupted suture closure of the gastric wall in rats. After two and four days, the stomach was excised and the bursting pressure was determined by gradually inflating the stomach with air. This procedure was followed by histological examinations.
Results:
The bursting pressure of the surgical wound was significantly higher two days after surgery in the h-LEH-treated rats in comparison to the control rats that received either empty liposome or RBC transfusion (P < 0.05). The three groups displayed similar bursting pressures four days after surgery. Histological examinations revealed less neutrophil infiltration, better granulation, and more macrophage infiltration in the h-LEH-treated rats after two days; however, these differences were no longer significant four days after surgery.
Conclusion:
The results suggest that h-LEH, but not a homologous transfusion or empty liposome, may accelerate early wound healing after a gastric incision and anastomosis in a rat model.
