Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Cells of the Epidermis01:24

Cells of the Epidermis

The epidermis is made of four or five layers of epithelial cells, depending on its location in the body. From deep to superficial, these layers are the stratum basale, stratum spinosum, stratum granulosum, stratum lucidum, and stratum corneum.
The cells in all these layers except the stratum basale are called keratinocytes, a type of cell that manufactures and stores the protein keratin. The keratinocytes in the stratum corneum are dead and regularly slough away, being replaced by cells from...
Renewal of Skin Epidermal Stem Cells01:12

Renewal of Skin Epidermal Stem Cells

The skin is divided into epidermis, dermis, and hypodermis, the skin's outermost, middle, and inner layers. The human epidermal layer regularly undergoes renewal, where old, dead cells are replaced by new cells. Epidermal stem cells or EpiSCs divide and differentiate to restore the lost cells. For the renewal process, some EpiSCs continuously self-renew. In contrast, few others differentiate into transit-amplifying cells, which later form prickle or spinous cells, followed by granular cells,...
Papillary Dermis01:11

Papillary Dermis

Dermis
The dermis might be considered the "core" of the integumentary system, as distinct from the epidermis and hypodermis. It contains blood and lymph vessels, nerves, and other structures, such as hair follicles and sweat glands. The dermis is made of two layers of connective tissue that comprise an interconnected mesh of elastin and collagenous fibers, produced by fibroblasts.
Papillary Layer
The papillary layer is made of loose, areolar connective tissue, which means the collagen and...
Tissue Renewal without Stem Cells01:23

Tissue Renewal without Stem Cells

After cellular or tissue damage, the resident stem cells present in the human body can locally repair and regenerate the damaged tissue or organ. However, even though some tissues do not have stem cells, they can repair and regenerate with the help of pre-existing cells. For example, beta cells of the pancreas and hepatocytes of the liver can divide to renew and regenerate the tissue. Here, both cell division and cell death are well regulated by homeostasis.
However, failure of such a system...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Nano-biotechnology: carbon nanofibres as improved neural and orthopaedic implants.

Nanotechnology·2021
Same author

Ciprofloxacin-Loaded Gold Nanoparticles against Antimicrobial Resistance: An In Vivo Assessment.

Nanomaterials (Basel, Switzerland)·2021
Same author

Improving the self-assembly of bioresponsive nanocarriers by engineering doped nanocarbons: a computational atomistic insight.

Scientific reports·2021
Same author

A Novel Para-Amino Salicylic Acid Magnesium Layered Hydroxide Nanocomposite Anti-Tuberculosis Drug Delivery System with Enhanced in vitro Therapeutic and Anti-Inflammatory Properties.

International journal of nanomedicine·2021
Same author

The promising use of nano-molecular imprinted templates for improved SARS-CoV-2 detection, drug delivery and research.

Journal of nanobiotechnology·2021
Same author

Calcium-based nanomaterials and their interrelation with chitosan: optimization for pCRISPR delivery.

Journal of nanostructure in chemistry·2021
Same journal

Superelastic Ti-Zr-Nb-Sn Thin Films Fabricated by Magnetron Sputtering: Biocompatibility and Bacterial Biofilm Formation Assessment for Orthopedic Applications.

Journal of biomedical materials research. Part A·2026
Same journal

Edible Sulfonated Soy Protein Microcarriers for Cultivated Meat Cell Expansion.

Journal of biomedical materials research. Part A·2026
Same journal

ROS-Responsive Quercetin Nanoparticles Improve the Prognosis of Traumatic Brain Injury by Inhibiting Aberrant Nrf2-Keap1 Signaling Pathway Activation.

Journal of biomedical materials research. Part A·2026
Same journal

Cellular Insights Into Proangiogenic Activation in Fibroblast and Endothelial Cells by Dual Drug-Loaded Emulsion Electrospun Nanofibers for Enhanced Tissue Regeneration.

Journal of biomedical materials research. Part A·2026
Same journal

Biomimetic Collagen Scaffolds Natural Cross-Linking Strategies via Transglutaminase and Methylglyoxal for Skin Repair.

Journal of biomedical materials research. Part A·2026
Same journal

Granular Hydrogel Composites for Noninvasive Optical Biosensing.

Journal of biomedical materials research. Part A·2026
See all related articles

Related Experiment Video

Updated: Jun 4, 2026

Evaluation of Keratinocyte Proliferation on Two- and Three-dimensional Type I Collagen Substrates
08:19

Evaluation of Keratinocyte Proliferation on Two- and Three-dimensional Type I Collagen Substrates

Published on: April 22, 2019

Nanostructured titanium promotes keratinocyte density.

Melanie A Zile1, Sabrina Puckett, Thomas J Webster

  • 1Department of Biomedical Engineering, College of Engineering, Boston University, Boston, Massachusetts 02215.

Journal of Biomedical Materials Research. Part A
|February 15, 2011
PubMed
Summary
This summary is machine-generated.

Nanoscale surface features on titanium implants enhance skin cell growth, potentially reducing infection and improving orthopedic implant success. This research explores titanium

Keywords:
FGF-2keratinocytenanotechnologyorthopedicsskintitanium

More Related Videos

An Innovative 3D-Printed Insert Designed to Enable Straightforward 2D and 3D Cell Cultures
08:17

An Innovative 3D-Printed Insert Designed to Enable Straightforward 2D and 3D Cell Cultures

Published on: January 6, 2023

Establishing a High Throughput Epidermal Spheroid Culture System to Model Keratinocyte Stem Cell Plasticity
10:03

Establishing a High Throughput Epidermal Spheroid Culture System to Model Keratinocyte Stem Cell Plasticity

Published on: January 30, 2021

Related Experiment Videos

Last Updated: Jun 4, 2026

Evaluation of Keratinocyte Proliferation on Two- and Three-dimensional Type I Collagen Substrates
08:19

Evaluation of Keratinocyte Proliferation on Two- and Three-dimensional Type I Collagen Substrates

Published on: April 22, 2019

An Innovative 3D-Printed Insert Designed to Enable Straightforward 2D and 3D Cell Cultures
08:17

An Innovative 3D-Printed Insert Designed to Enable Straightforward 2D and 3D Cell Cultures

Published on: January 6, 2023

Establishing a High Throughput Epidermal Spheroid Culture System to Model Keratinocyte Stem Cell Plasticity
10:03

Establishing a High Throughput Epidermal Spheroid Culture System to Model Keratinocyte Stem Cell Plasticity

Published on: January 30, 2021

Area of Science:

  • Biomaterials Science
  • Tissue Engineering
  • Orthopedic Surgery

Background:

  • Transcutaneous orthopedic implant infections reduce success rates.
  • Reducing bacterial colonization and promoting skin cell growth are key to preventing infections.
  • Previous research shows titanium's nanofeatures reduce bacterial adhesion and support bone cells.

Purpose of the Study:

  • To evaluate keratinocyte (skin cell) functions on titanium with nanoscale surface modifications.
  • To investigate the effect of fibroblast growth factor-2 (FGF-2) on keratinocyte behavior on these surfaces.

Main Methods:

  • Created nanometer-sized topographical features on titanium (Ti) using anodization (nanotubular) and electron beam evaporation (nanorough).
  • Cultured keratinocytes on conventional (nanosmooth) Ti, nanotubular Ti, and nanorough Ti.
  • Functionalized some Ti surfaces with FGF-2 to assess its impact on keratinocyte density.

Main Results:

  • Nanotubular and nanorough Ti surfaces significantly promoted keratinocyte density compared to conventional Ti.
  • FGF-2 further increased keratinocyte density on all tested Ti surfaces.
  • The highest keratinocyte density was observed on nanorough and nanotubular Ti surfaces functionalized with FGF-2 after 5 days.

Conclusions:

  • Specific nanoscale titanium topographies can enhance keratinocyte density.
  • Combining nanostructured titanium with FGF-2 maximizes skin cell growth.
  • These findings suggest nanostructured titanium's potential for improving transcutaneous orthopedic implants by promoting skin integration and reducing infection.