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Related Concept Videos

Electrospray Ionization (ESI) Mass Spectrometry01:12

Electrospray Ionization (ESI) Mass Spectrometry

Higher molecular weight biomolecules are nonvolatile compounds that may decompose before ionizing or vaporizing during mass analysis with conventional electron impact ionization methods. Accordingly, electrospray ionization (ESI) is the favored method for vaporizing and ionizing biomolecules as it circumvents rapid fragmentation and enables the recording of mass signals for the entire biomolecule.
ESI utilizes electrical energy to transfer ions from the liquid phase of the sample into the...
Mass Spectrometry: Complex Analysis01:21

Mass Spectrometry: Complex Analysis

Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
Capillary Electrophoresis: Instrumentation01:20

Capillary Electrophoresis: Instrumentation

Capillary electrophoresis instrumentation typically consists of several key components. A high-voltage power supply generates the electric field necessary for the separation by connecting to an anode (the positively charged electrode) and a cathode (the negatively charged electrode) located in buffer reservoirs at each end of the capillary tube. The system includes a sample vial, a fused silica capillary tube coated with polyimide for mechanical strength through which the sample components...
Capillary Electrophoresis: Applications01:30

Capillary Electrophoresis: Applications

Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
MALDI-TOF Mass Spectrometry01:19

MALDI-TOF Mass Spectrometry

Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.Matrix-assisted laser desorption ionization (MALDI) is a commonly...
Electrophoresis: Overview01:20

Electrophoresis: Overview

Electrophoresis is a powerful analytical separation technique that relies on the differential migration of charged species when subjected to an electric field. The core strength of electrophoresis lies in its ability to separate high-molecular-weight species in complex mixtures. It has found widespread use in biochemistry, molecular biology, and analytical chemistry, allowing the separation of compounds like amino acids, nucleotides, carbohydrates, and proteins with excellent resolution.
There...

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Imaging of Biological Tissues by Desorption Electrospray Ionization Mass Spectrometry
06:21

Imaging of Biological Tissues by Desorption Electrospray Ionization Mass Spectrometry

Published on: July 12, 2013

Interfacing capillary-based separations to mass spectrometry using desorption electrospray ionization.

Griffin K Barbula1, Samir Safi, Konstantin Chingin

  • 1Department of Chemistry, Stanford University, Stanford, California 94305, United States.

Analytical Chemistry
|February 16, 2011
PubMed
Summary
This summary is machine-generated.

A new desorption electrospray ionization (DESI) method overcomes salt and detergent interference in capillary electrophoresis and micellar electrokinetic chromatography coupled with mass spectrometry. This technique enhances signal and reduces mass spectrometer contamination for improved hybrid analysis.

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Imaging of Biological Tissues by Desorption Electrospray Ionization Mass Spectrometry
06:21

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Published on: July 12, 2013

Sheathless Capillary Electrophoresis&#8211;Mass Spectrometry for Metabolic Profiling of Biological Samples
07:46

Sheathless Capillary Electrophoresis–Mass Spectrometry for Metabolic Profiling of Biological Samples

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High-throughput and Comprehensive Drug Surveillance Using Multisegment Injection-Capillary Electrophoresis-Mass Spectrometry
10:17

High-throughput and Comprehensive Drug Surveillance Using Multisegment Injection-Capillary Electrophoresis-Mass Spectrometry

Published on: April 23, 2019

Area of Science:

  • Analytical Chemistry
  • Separation Science
  • Mass Spectrometry

Background:

  • Capillary-based separations coupled with mass spectrometry (MS) are powerful for chemical analysis.
  • Electrospray ionization (ESI) is commonly used but suffers from ion suppression and contamination caused by salts and detergents in mobile phases.
  • These limitations hinder sensitive and reliable MS detection in electrokinetic separations.

Purpose of the Study:

  • To develop a novel method using desorption electrospray ionization (DESI) to mitigate ion suppression and inlet contamination in capillary-based separations coupled with MS.
  • To demonstrate the effectiveness of DESI in analyzing samples containing salts and detergents, commonly found in electrokinetic separation buffers.

Main Methods:

  • Effluent from capillary columns was deposited onto a rotating, paper-covered Teflon disk, spatially encoding the electropherogram.
  • Desorption electrospray ionization (DESI) was employed to ionize the surface-deposited analytes.
  • The method was tested using capillary electrophoresis (CE) of rhodamine dyes and micellar electrokinetic chromatography (MEKC) of cardiac drugs.

Main Results:

  • DESI preferentially ionized surface-deposited analytes, significantly reducing the negative impact of salts and detergents on MS signal.
  • Ion suppression and mass spectrometer inlet contamination were minimized in both CE and MEKC experiments.
  • Successful analysis of rhodamine dyes and cardiac drugs was achieved despite the presence of buffer salts and SDS.

Conclusions:

  • The developed DESI-based surface deposition technique effectively overcomes the limitations of traditional ESI for capillary-based separations in MS.
  • This novel approach offers a robust solution for analyzing complex samples containing interfering salts and detergents.
  • The method enhances analytical performance and reliability in hybrid separation-mass spectrometry techniques.