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Synthesis and characterization of Au102(p-MBA)44 nanoparticles
Yael Levi-Kalisman1, Pablo D Jadzinsky, Nir Kalisman
1Department of Structural Biology, Stanford University School of Medicine, Stanford, California 94305, USA.
Abstract:
The synthesis of Au(102)(p-MBA)(44) nanoparticles on a preparative scale in high yield is described. Various analytical methods are shown to give results consistent with the composition and known structure of the particles, showing the preparation is essentially homogeneous, and attesting to the validity of the methods as well. Derivatization of the particles with proteins and DNA is demonstrated, and conditions are described for imaging individual particles by cryo-EM at low electron dose, close to focus, conditions optimal for recording high-resolution details.
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