Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Cohesins

Cohesins

Cohesin protein complexes are a molecular glue that holds two sister chromatids together. They play an important role both in mitosis and meiosis. In mitosis, all cohesin complexes present on the chromosomes are removed before the start of the anaphase stage.
Cohesin complexes in Meiotic Division
Meiosis involves two distinct rounds of chromosomal segregation and cell divisions— Meiosis I followed by Meiosis II – producing four daughter cells. Meiosis I includes the separation of homologous...

Cohesins

Cohesins

Cohesin protein complexes are a molecular glue that holds two sister chromatids together. They play an important role both in mitosis and meiosis. In mitosis, all cohesin complexes present on the chromosomes are removed before the start of the anaphase stage.
Cohesin complexes in Meiotic Division
Meiosis involves two distinct rounds of chromosomal segregation and cell divisions— Meiosis I followed by Meiosis II – producing four daughter cells. Meiosis I includes the separation of homologous...

Separation of Sister Chromatids

Separation of Sister Chromatids

At the transition from prophase to metaphase, there is a reduction in cohesion along the chromosomal arms, resulting in the resolution of sister chromatids. However, residual cohesin connections remain to hold the sister chromatids together until the transition from metaphase to anaphase. The residual connection prevents any premature separation of sister chromatids, blocking the risks of aneuploidy within the daughter cells.
At the onset of anaphase, separase, a proteolytic enzyme, is...

Separation of Sister Chromatids

Separation of Sister Chromatids

At the transition from prophase to metaphase, there is a reduction in cohesion along the chromosomal arms, resulting in the resolution of sister chromatids. However, residual cohesin connections remain to hold the sister chromatids together until the transition from metaphase to anaphase. The residual connection prevents any premature separation of sister chromatids, blocking the risks of aneuploidy within the daughter cells.
At the onset of anaphase, separase, a proteolytic enzyme, is...

Condensins

Condensins

Condensins are large protein complexes that use ATP to fuel the assembly of chromosomes during mitosis. They transform the tangled, shapeless mass of post-interphase DNA into individualized chromosomes by compacting, organizing, and segregating chromosomal DNA.
The plant and animal cells contain two types of condensin complexes—condensin I and condensin II. Both complexes have five subunits: two SMC (Structural Maintenance of Chromosomes) subunits, a kleisin subunit, and two HEAT-repeat...

Condensins

Condensins

Condensins are large protein complexes that use ATP to fuel the assembly of chromosomes during mitosis. They transform the tangled, shapeless mass of post-interphase DNA into individualized chromosomes by compacting, organizing, and segregating chromosomal DNA.
The plant and animal cells contain two types of condensin complexes—condensin I and condensin II. Both complexes have five subunits: two SMC (Structural Maintenance of Chromosomes) subunits, a kleisin subunit, and two HEAT-repeat...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Investigations into multiple fission yeast chromosome size determinants.

Journal of cell science·2026

Same author

Why is your separase such a big protease?

Science advances·2025

Same author

Biochemical Reconstitution of Replication-Coupled Cohesin Acetylation.

Methods in molecular biology (Clifton, N.J.)·2025

Same author

Progressive chromosome shape changes during cell divisions.

EMBO reports·2025

Same author

Biochemical reconstitution of sister chromatid cohesion establishment during DNA replication.

Molecular cell·2025

Same author

Replisome passage through the cohesin ring.

Cell·2025

Search research articles

Related Experiment Video

Updated: Jun 4, 2026

Examination of Mitotic and Meiotic Fission Yeast Nuclear Dynamics by Fluorescence Live-cell Microscopy

Examination of Mitotic and Meiotic Fission Yeast Nuclear Dynamics by Fluorescence Live-cell Microscopy

Published on: June 24, 2019

Cohesin loading and sliding.

Maria T Ocampo-Hafalla¹, Frank Uhlmann

¹Chromosome Segregation Laboratory, Cancer Research UK London Research Institute, Lincoln's Inn Fields Laboratories, 44 Lincoln's Inn Fields, London WC2A 3LY, UK.

Journal of Cell Science

|February 16, 2011

Summary

This summary is machine-generated.

Cohesin, a ring-like complex, is vital for chromosomal stability and gene regulation. Its loading onto DNA by the Scc2-Scc4 complex and subsequent chromosome interactions are key to its functions.

More Related Videos

Using Fluorescence In Situ Hybridization (FISH) to Monitor the State of Arm Cohesion in Prometaphase and Metaphase I Drosophila Oocytes

Using Fluorescence In Situ Hybridization (FISH) to Monitor the State of Arm Cohesion in Prometaphase and Metaphase I Drosophila Oocytes

Published on: December 6, 2017

Cargo Loading onto Kinesin Powered Molecular Shuttles

Cargo Loading onto Kinesin Powered Molecular Shuttles

Published on: November 3, 2010

Related Experiment Videos

Last Updated: Jun 4, 2026

Examination of Mitotic and Meiotic Fission Yeast Nuclear Dynamics by Fluorescence Live-cell Microscopy

Examination of Mitotic and Meiotic Fission Yeast Nuclear Dynamics by Fluorescence Live-cell Microscopy

Published on: June 24, 2019

Using Fluorescence In Situ Hybridization (FISH) to Monitor the State of Arm Cohesion in Prometaphase and Metaphase I Drosophila Oocytes

Using Fluorescence In Situ Hybridization (FISH) to Monitor the State of Arm Cohesion in Prometaphase and Metaphase I Drosophila Oocytes

Published on: December 6, 2017

Cargo Loading onto Kinesin Powered Molecular Shuttles

Cargo Loading onto Kinesin Powered Molecular Shuttles

Published on: November 3, 2010

Area of Science:

Molecular Biology
Genetics
Cell Biology

Background:

Cohesin is essential for sister chromatid cohesion and chromosomal stability.
It plays roles in gene regulation and development across various organisms.
The precise mechanisms of cohesin's chromosomal association remain unclear.

Purpose of the Study:

To critically review evidence for cohesin loading onto chromosomes.
To discuss the implications of cohesin localization studies for its functions.
To elucidate the mechanisms of cohesin-DNA interactions.

Main Methods:

Literature review of cohesin loading and localization studies.
Analysis of cohesin's role in sister chromatid cohesion and gene expression.
Discussion of related complexes like condensin and Smc5-Smc6.

Main Results:

Cohesin binding to chromosomes is dependent on the Scc2-Scc4 complex (loading factor).
Cohesin exhibits functions beyond sister chromatid cohesion, including gene regulation.
Evidence suggests cohesin may slide along chromosomes after loading.

Conclusions:

Understanding cohesin loading and movement is crucial for its diverse roles.
Further research is needed to fully elucidate cohesin's mechanisms in chromosome biology.
Cohesin's functions in gene regulation and development highlight its broad importance.