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Updated: Jun 4, 2026

Isolation of Low Endotoxin Content Extracellular Vesicles Derived from Cancer Cell Lines
Published on: February 17, 2023
Selective removal of endotoxin from a DNA solution by cross-linked cyclodextrin beads
Masayo Sakata1, Kana Yoshimura, Itsumi Sakamoto
1Department of Applied Chemistry & Biochemistry, Graduate School of Science and Technology, Kumamoto University, 2-39-1 Kurokami, Kumamoto 860-8555, Japan. msakata@gpo.kumamoto-u.ac.jp
Abstract:
The removal of lipopolysaccharide (LPS) from a contaminated DNA solution was achieved using cross-linked cyclodextrin (CyD polymer) beads as LPS adsorbents. The LPS-removing activity of the β- and γ-CyD polymer beads was compared with that of common cationic LPS adsorbents. The γ-CyD polymer beads selectively removed LPS from a DNA solution (50 µg mL(-1), pH 6, ionic strength μ = 0.2) containing natural LPS (15 EU mL(-1)), without the adsorption of DNA. The adsorptions of LPS and DNA were 85% and <1%, respectively.
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