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B Cell Activation and Differentiation01:24

B Cell Activation and Differentiation

The adaptive immune response, a sophisticated defense mechanism, relies on the activation and differentiation of B lymphocytes, or B cells. These processes enable our bodies to mount a tailored response against specific pathogens such as bacteria, free virus particles, toxins, and parasites.
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Differentiation of Functional Osteoclasts from Human Peripheral Blood CD14+ Monocytes
11:52

Differentiation of Functional Osteoclasts from Human Peripheral Blood CD14+ Monocytes

Published on: January 27, 2023

Osteoclast activity modulates B-cell development in the bone marrow.

Anna Mansour1, Adrienne Anginot, Stéphane J C Mancini

  • 1CNRS, GEPITOS, UMR 6235, Faculté de Médecine, 06100 Nice, France.

Cell Research
|February 16, 2011
PubMed
Summary
This summary is machine-generated.

Osteoclasts (OCLs) regulate B-cell development in bone marrow by influencing the bone microenvironment and osteoblast activity. This study reveals how OCLs control B-cell retention within their niches.

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Osteoclast Derivation from Mouse Bone Marrow
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Last Updated: Jun 4, 2026

Differentiation of Functional Osteoclasts from Human Peripheral Blood CD14+ Monocytes
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Differentiation of Functional Osteoclasts from Human Peripheral Blood CD14+ Monocytes

Published on: January 27, 2023

Osteoclast Derivation from Mouse Bone Marrow
06:17

Osteoclast Derivation from Mouse Bone Marrow

Published on: November 6, 2014

Area of Science:

  • Immunology
  • Bone Biology
  • Hematopoiesis

Background:

  • B-cell development relies on bone marrow stromal cell interactions.
  • The specific role of osteoclasts (OCLs) in B lymphopoiesis is not well understood.
  • Osteopetrosis, characterized by B lymphocytopenia, suggests OCLs influence B-cell production.

Purpose of the Study:

  • To investigate the role of osteoclasts (OCLs) in regulating B-cell development within the bone marrow microenvironment.
  • To determine if OCL activity impacts B lymphopoiesis and the maintenance of B-cell niches.

Main Methods:

  • Restored OCL function in osteopetrotic mice (oc/oc) via dendritic cell transfer.
  • Induced osteopetrosis in normal mice using zoledronic acid (ZA), a bone resorption inhibitor.
  • Assessed B-cell numbers, differentiation, proliferation, and apoptosis, along with stromal cell gene expression (CXCL12, IL-7) and osteoblastic engagement.

Main Results:

  • Restoring OCL function in oc/oc mice normalized bone phenotype and B-cell development.
  • ZA-induced osteopetrosis led to decreased B-cell numbers in bone marrow.
  • ZA treatment reduced CXCL12 and IL-7 expression in stromal cells and decreased osteoblastic engagement, impacting B lymphopoiesis and causing B-cell progenitor retention outside niches.

Conclusions:

  • Osteoclasts (OCLs) play a crucial role in modulating B-cell development within the bone marrow.
  • OCLs control the bone microenvironment and osteoblast fate, thereby regulating B-cell retention in their niches.
  • These findings offer new insights into the regulation of B-cell homeostasis by osteoclast activity.