Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Multipotency and Niche of Bulge Stem Cell

Multipotency and Niche of Bulge Stem Cell

A hair follicle or HF is a small part of the skin that produces the hair shaft. Paul Gerson Unna was the first to observe a bulge in the human hair follicle's outer root sheath (ORS). The bulge is present between the sebaceous gland and the arrector pili muscle and is the niche for hair follicle stem cells (HFSCs). The bulge is also a niche for melanocyte stem cells, and their loss results in graying of hair. The HFSCs express Sox9 and Lhx2, which help them maintain stemness and prevent...

Lineage Commitment

Lineage Commitment

Commitment is the process whereby stem cells:

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Exercise frequency and physician burnout among colorectal surgeons in the USA: should we be worried?

Techniques in coloproctology·2026

Same author

Factors affecting the efficacy of nonsurgical therapy of peri-implantitis.

Periodontology 2000·2026

Same author

Gingival and periodontal diseases and conditions in children and adolescents: consensus report.

European archives of paediatric dentistry : official journal of the European Academy of Paediatric Dentistry·2026

Same author

From Disease to Illness: Reframing Periodontitis Through an Anthropological Lens.

Journal of periodontal research·2025

Same author

The efficacy of biologic factors on the surgical therapy of periodontal suprabony defects: A systematic review and network meta-analysis of randomized clinical trials.

Periodontology 2000·2025

Same author

Ideal soft tissue graft in periodontal and peri-implant applications: A scoping review.

Periodontology 2000·2025

Search research articles

Related Experiment Video

Updated: Jun 4, 2026

Phenotypic Analysis and Isolation of Murine Hematopoietic Stem Cells and Lineage-committed Progenitors

Phenotypic Analysis and Isolation of Murine Hematopoietic Stem Cells and Lineage-committed Progenitors

Published on: July 8, 2012

A procedure for identifying stem cell compartments with multi-lineage differentiation potential.

Harsh D Amin¹, Irwin Olsen, Jonathan Knowles

¹Division of Biomaterials and Tissue Engineering, UCL Eastman Dental Institute, University College London, London, UK.

|February 16, 2011

Summary

Researchers developed new methods to identify adult stem cells from human ligament tissue. These techniques assess the multi-lineage potential for tissue repair and regeneration, advancing stem cell research.

More Related Videos

Identification and Isolation of Oligopotent and Lineage-committed Myeloid Progenitors from Mouse Bone Marrow

Identification and Isolation of Oligopotent and Lineage-committed Myeloid Progenitors from Mouse Bone Marrow

Published on: July 29, 2018

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations

Published on: October 25, 2018

Related Experiment Videos

Last Updated: Jun 4, 2026

Phenotypic Analysis and Isolation of Murine Hematopoietic Stem Cells and Lineage-committed Progenitors

Phenotypic Analysis and Isolation of Murine Hematopoietic Stem Cells and Lineage-committed Progenitors

Published on: July 8, 2012

Identification and Isolation of Oligopotent and Lineage-committed Myeloid Progenitors from Mouse Bone Marrow

Identification and Isolation of Oligopotent and Lineage-committed Myeloid Progenitors from Mouse Bone Marrow

Published on: July 29, 2018

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations

Published on: October 25, 2018

Area of Science:

Regenerative Medicine
Stem Cell Biology
Tissue Engineering

Background:

Adult stem cells hold promise for tissue repair and regeneration.
Identifying and characterizing adult stem cell populations is challenging due to limited methodologies for assessing multi-lineage potential.

Purpose of the Study:

To develop and validate a combination of methods for identifying adult stem cell compartments.
To assess the multi-lineage differentiation potential of cells from adult human ligament tissue in vitro.

Main Methods:

Utilized adult human ligament tissue as a model system.
Employed reverse transcription polymerase chain reaction (RT-PCR) to analyze lineage-associated gene expression.
Applied histological, immunological, and morphological criteria to evaluate in vitro differentiation.

Main Results:

Successfully identified adult stem cell compartments within human ligament tissue.
Demonstrated the multi-lineage differentiation capacity of these cells, including osteogenesis, adipogenesis, chondrogenesis, myogenesis, vasculogenesis, angiogenesis, neurogenesis, and gliogenesis.
Validated the combination of molecular and cellular assays for stem cell characterization.

Conclusions:

The developed methodology enables robust identification and characterization of adult stem cells.
This approach facilitates the assessment of multi-lineage potential, crucial for regenerative medicine applications.
Adult human ligament tissue serves as a valuable source for stem cells with broad differentiation capabilities.