IgLONs form heterodimeric complexes on forebrain neurons
Christine J McNamee1, Sahar Youssef, Diana Moss
1Department of Human Anatomy and Cell Biology, Liverpool University, UK.
Cell Biochemistry and Function
|February 16, 2011
Summary
The Immunoglobulin-like domain containing, LGI family (IgLONs) proteins form heterodimeric complexes. This study demonstrates IgLONs are the first GPI-anchored glycoproteins shown to form heterodimers in the cell membrane.
Area of Science:
- Neuroscience
- Cell Biology
- Molecular Biology
Background:
- Immunoglobulin-like domain containing, LGI family (IgLONs) are GPI-anchored cell adhesion molecules.
- IgLONs regulate neurite outgrowth, synaptogenesis, and may function as tumor suppressors.
- A heterodimeric complex termed DIgLON has been recently proposed.
Purpose of the Study:
- To investigate the formation of heterodimeric complexes within the IgLON family.
- To determine if IgLONs form complexes with each other in neurons.
- To establish if IgLONs are the first GPI-anchored glycoproteins shown to form heterodimers.
Main Methods:
- Utilized neurons isolated from chick forebrain.
- Co-expressed all six combinations of IgLONs.
- Performed antibody-patching experiments on forebrain neurons.
Main Results:
- Neurons co-expressed all six IgLON combinations with highly correlated fluorescence intensity.
- Antibody-patching confirmed complex formation between IgLON pairs.
- No complex formation was observed between unrelated GPI-anchored glycoproteins.
Conclusions:
- IgLONs are the first identified GPI-anchored glycoprotein family to form heterodimeric complexes.
- IgLON heterodimerization occurs within the plane of the cell membrane.
- This finding provides novel insights into the molecular mechanisms of IgLON function in neuronal development and potentially in cancer.
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