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Updated: Jun 4, 2026
Chromatographic Fingerprinting by Template Matching for Data Collected by Comprehensive Two-Dimensional Gas Chromatography
Published on: September 2, 2020
[HPLC fingerprints of processed products from Sinapis alba]
Cun Zhang1, Li Li, Yongqing Xiao
1Institute of Chinese Materia Madica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
Objective:
To establish the HPLC fingerprint method and compare the changes of chemical compositions of the processed products from Sinapis alba.
Method:
The procedure of HPLC analysis was performed on a agilent TC-C18 (2) column at 35 degrees C with the acetonitrile -0.1% phosphoric acid in gradient elution as the mobile phase. The detection wavelength was set at 254 nm, and the flow rate was 1.0 mL x min(-1). The similarity evaluation system for chromatographic fingerprint of traditional Chinese medicine (Version 2004 A) was applied to analyze the similarity.
Result:
The average similarities among the processed products were over 0.96, the standard HPLC fingerprint and five main chromatographic peaks with the isolated compounds was obtained and identified.
Conclusion:
The established methods with two solvents are suitable for the HPLC fingerprints determination which elementary elucidate the scientific intensions of breaking the enzyme for glycosides.
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