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Updated: Jun 4, 2026
Real-Time Polymerase Chain Reaction-Based Detection and Quantification of Hepatitis B Virus DNA
Published on: December 15, 2023
Establishment of a new method for the detection of the affinity of antibody to hepatitis B e antigen by a routine
1Department of Clinical Immunology, Dalian Medical University, Dalian, China. liuhui60@mail.dlptt.ln.cn
Background:
The commonly used quantitative analyses for specific antibodies only involve measurement of the total antibody activity, and the binding affinity is rarely analyzed. We established a method for the detection of the affinity of the antibody to hepatitis B e antigen (anti-HBe).
Methods:
A method to detect anti-HBe affinity was established by using a routine quantitative system (Architect Analyzer). Serum samples were collected from hepatitis B patients with anti-HBe. On the basis of the other serologic markers in the samples, patients were divided into HBsAg positive and anti-HBs positive groups. The total anti-HBe activity and anti-HBe affinity were each measured.
Results:
The affinity of anti-HBe in the anti-HBs positive group was higher than that in the HBsAg positive group (P<0.05); conversely, the total anti-HBe activity in the anti-HBs positive group was lower than that in the HBsAg positive group (P<0.05).
Conclusions:
The anti-HBe affinity arising from an immune response to HBV infection may vary significantly between different situations. This new approach will be suitable for determining antibody affinity in clinical laboratories.
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