Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jun 4, 2026

Inducing Apical Periodontitis in Mice
10:26

Inducing Apical Periodontitis in Mice

Published on: August 6, 2019

PHEX neutralizing agent inhibits dentin formation in mouse tooth germ.

Hongbing Lv1, Sheng Fu, Gancha Wu

  • 1Department of Endodontics, School and Hospital of Stomatology, Fujian Medical University, Fuzhou, China.

Tissue & Cell
|February 18, 2011
PubMed
Summary

PHEX gene mutations cause hypophosphatemic rickets. This study found that reduced PHEX protein expression in mice significantly inhibits dentin formation, while enamel formation is only slightly affected.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Periodontitis aggravates bone loss through gut microbiota alterations in ovariectomized mice.

NPJ biofilms and microbiomes·2026
Same author

Periodontitis-associated gut microbiota disrupts glucose homeostasis through SCFAs depletion and inflammation in germ-free mice.

BMC oral health·2026
Same author

Membrane and bioenergetic dysfunction in Parkinson's disease with REM sleep behavior disorder: A <sup>31</sup>P and <sup>1</sup>H MR study.

NeuroImage·2026
Same author

The Effect of miR-129-5p on the Proliferation and Osteogenic Differentiation of Human Dental Pulp Stem Cells.

International dental journal·2026
Same author

Gut microbiota dysbiosis and metabolic alterations in rheumatoid arthritis: a barrier to periodontal repair.

RMD open·2026
Same author

Asymptomatic Abnormalities in the Knee, Shoulder, and Ankle Joints of Collegiate Athletes: A Cross-Sectional MRI-Based Comparative Study.

Diagnostics (Basel, Switzerland)·2026

Area of Science:

  • Biochemistry
  • Genetics
  • Developmental Biology

Background:

  • Mutations in the phosphate-regulating gene with homologies to endopeptidases on the X-chromosome (PHEX) cause X-linked hypophosphatemic rickets.
  • This condition is characterized by impaired bone and dentin mineralization.
  • The role of PHEX in tooth development, particularly in enamel and dentin formation, requires further investigation.

Purpose of the Study:

  • To investigate the role of PHEX in the formation of enamel and dentin during tooth development.
  • To analyze the effects of PHEX modulation on ameloblasts and odontoblasts.

Main Methods:

  • Pregnant mice were injected with PHEX antibody on embryonic day 15 (E15).
  • Mallory trichrome staining was used to assess tissue structure.

More Related Videos

Development of a Direct Pulp-capping Model for the Evaluation of Pulpal Wound Healing and Reparative Dentin Formation in Mice
07:07

Development of a Direct Pulp-capping Model for the Evaluation of Pulpal Wound Healing and Reparative Dentin Formation in Mice

Published on: January 12, 2017

Microhardness Measurements on Tooth and Alveolar Bone in Rodent Oral Disease Models
06:16

Microhardness Measurements on Tooth and Alveolar Bone in Rodent Oral Disease Models

Published on: April 26, 2024

Related Experiment Videos

Last Updated: Jun 4, 2026

Inducing Apical Periodontitis in Mice
10:26

Inducing Apical Periodontitis in Mice

Published on: August 6, 2019

Development of a Direct Pulp-capping Model for the Evaluation of Pulpal Wound Healing and Reparative Dentin Formation in Mice
07:07

Development of a Direct Pulp-capping Model for the Evaluation of Pulpal Wound Healing and Reparative Dentin Formation in Mice

Published on: January 12, 2017

Microhardness Measurements on Tooth and Alveolar Bone in Rodent Oral Disease Models
06:16

Microhardness Measurements on Tooth and Alveolar Bone in Rodent Oral Disease Models

Published on: April 26, 2024

  • Immunohistochemistry was employed to determine the spatiotemporal distribution and expression levels of PHEX protein in tooth germs at various developmental stages (E16, E18, P1, P3).

    • Main Results:

    • PHEX antibody treatment led to irregular arrangements of ameloblasts and odontoblasts.
    • Dentin formation and odontoblast differentiation were significantly inhibited.
    • PHEX protein was detected in the inner enamel epithelium, ameloblasts, and odontoblasts, with expression levels changing during development.
    • A decrease in PHEX expression was correlated with suppressed dentin formation, whereas enamel formation showed only minor effects.

    Conclusions:

    • PHEX plays a crucial role in regulating dentin formation during tooth development.
    • Reduced PHEX expression primarily impacts dentinogenesis, with a lesser effect on amelogenesis.
    • These findings highlight PHEX as a key factor in maintaining proper dentin development.