Assessing a novel room temperature DNA storage medium for forensic biological samples

Area of Science:

• Forensic Science
• Molecular Biology
• Biotechnology

Background:

• Preserving biological evidence integrity is crucial for forensic analysis.
• DNA testing often occurs after sample collection, necessitating stable storage.
• Retesting stored DNA samples is vital for confirmation and future analyses.

Purpose of the Study:

• To evaluate SampleMatrix™ (SM), a novel room temperature DNA storage medium.
• To assess SM's efficacy in stabilizing and protecting DNA samples under various conditions.
• To determine potential inhibitory effects of SM on DNA analysis.

Main Methods:

• Human genomic DNA samples (0.0625-200 ng) were stored dry in SM for up to 1 year.
• Samples were subjected to ambient laboratory conditions and freeze-thaw cycles.
• DNA recovery was quantified using qPCR and gel electrophoresis; STR analyses assessed DNA profile quality.

Main Results:

• No significant quality differences were observed between SM-stored and frozen samples.
• SM provided superior DNA recovery compared to freezer storage for long-term and low-concentration samples.
• No amplification inhibition was detected, even at higher SM concentrations; complete DNA profiles were obtained.

Conclusions:

• SampleMatrix™ effectively stabilizes DNA at ambient temperatures, simplifying storage requirements.
• This technology offers a significant advantage for forensic evidence and other DNA storage applications.
• SM enhances DNA recovery and preserves sample integrity for reliable downstream analyses.