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Related Concept Videos

Spindle Assembly02:50

Spindle Assembly

Spindle assembly occurs through three, often coexisting, pathways – the centrosome-mediated pathway, the chromatin-mediated pathway, and the microtubule-mediated pathway – collectively contributing to form a robust spindle apparatus.
In most cells, centrosomes are the primary microtubule nucleation centers. In the centrosome-mediated pathway, the G2-prophase transition triggers centrosome maturation and increased microtubule nucleation. Progressive nucleation results in a microtubule array...
Spindle Assembly02:50

Spindle Assembly

Spindle assembly occurs through three, often coexisting, pathways – the centrosome-mediated pathway, the chromatin-mediated pathway, and the microtubule-mediated pathway – collectively contributing to form a robust spindle apparatus.
In most cells, centrosomes are the primary microtubule nucleation centers. In the centrosome-mediated pathway, the G2-prophase transition triggers centrosome maturation and increased microtubule nucleation. Progressive nucleation results in a microtubule array...
Attachment of Sister Chromatids02:57

Attachment of Sister Chromatids

As cells progress into mitosis, the nuclear envelope breaks down, and the condensed chromosomes are exposed to the array of bipolar microtubules of the mitotic spindle. The kinetochore, a large, disc-shaped protein complex, is present at the centromere region of the sister chromatids and acts as a binding site for the microtubules.  Usually, the plus-end of a single microtubule is embedded within the kinetochore. However, some kinetochores first establish lateral contact with the side-wall of a...
The Spindle Assembly Checkpoint02:19

The Spindle Assembly Checkpoint

The spindle assembly checkpoint is a molecular surveillance mechanism ensuring the fidelity of chromosome segregation during anaphase. The checkpoint monitors the completion of all the prerequisite steps before chromosome segregation to determine whether the segregation process should proceed or be delayed.
Many proteins function together to control the spindle assembly checkpoint. Mutations affecting these proteins may allow cells to proceed into anaphase prematurely, resulting in the...
The Spindle Assembly Checkpoint02:19

The Spindle Assembly Checkpoint

The spindle assembly checkpoint is a molecular surveillance mechanism ensuring the fidelity of chromosome segregation during anaphase. The checkpoint monitors the completion of all the prerequisite steps before chromosome segregation to determine whether the segregation process should proceed or be delayed.
Many proteins function together to control the spindle assembly checkpoint. Mutations affecting these proteins may allow cells to proceed into anaphase prematurely, resulting in the...
The Mitotic Spindle02:27

The Mitotic Spindle

The mitotic spindle—or spindle apparatus—is a eukaryotic, cytoskeletal structure made up of long protein fibers called microtubules. Formed during cell division, the spindle separates sister chromatids and moves them to opposite ends of a parental cell, where the now individual chromosomes are distributed to two daughter cell nuclei.
The bipolar configuration of the mitotic spindle facilitates chromosomal segregation, preparing the cell for division. One mechanism that ensures bipolar mitotic...

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Related Experiment Video

Updated: Jun 4, 2026

Real-Time Monitoring of Aurora kinase A Activation using Conformational FRET Biosensors in Live Cells
06:29

Real-Time Monitoring of Aurora kinase A Activation using Conformational FRET Biosensors in Live Cells

Published on: July 30, 2020

Aurora kinases and spindle assembly: variations on a common theme?

Vladimir Joukov1

  • 1Department of Cancer Biology, Dana-Farber Cancer Institute and Harvard Medical School, Boston, MA, USA. vladimir_joukov@dfci.harvard.edu

Cell Cycle (Georgetown, Tex.)
|February 18, 2011
PubMed
Summary

Researchers identified Cep192/Spd-2 as a novel activator of Aurora A kinase (AurA) at centrosomes. This discovery reveals a new mechanism for regulating AurA activity, crucial for cell division and microtubule organization.

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Related Experiment Videos

Last Updated: Jun 4, 2026

Real-Time Monitoring of Aurora kinase A Activation using Conformational FRET Biosensors in Live Cells
06:29

Real-Time Monitoring of Aurora kinase A Activation using Conformational FRET Biosensors in Live Cells

Published on: July 30, 2020

Reconstitution of Basic Mitotic Spindles in Spherical Emulsion Droplets
10:52

Reconstitution of Basic Mitotic Spindles in Spherical Emulsion Droplets

Published on: August 13, 2016

Live Cell Imaging to Assess the Dynamics of Metaphase Timing and Cell Fate Following Mitotic Spindle Perturbations
07:14

Live Cell Imaging to Assess the Dynamics of Metaphase Timing and Cell Fate Following Mitotic Spindle Perturbations

Published on: September 20, 2019

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Biochemistry

Background:

  • Aurora A kinase (AurA) is essential for cell division, regulating centrosome maturation and spindle assembly.
  • TPX2 is a known cofactor that targets AurA to microtubules and promotes its activation.
  • The precise mechanism of AurA regulation at centrosomes remained unclear, prompting the search for a specific activator.

Purpose of the Study:

  • To identify the proposed centrosome-specific activator of Aurora A kinase.
  • To elucidate the mechanism by which this activator regulates AurA activity at centrosomes.

Main Methods:

  • The study involved identifying and characterizing the interaction between AurA and potential activators at centrosomes.
  • Investigated the role of Cep192/Spd-2 in AurA targeting and activation using biochemical and cell biological approaches.

Main Results:

  • Cep192/Spd-2 was identified as a key centrosome-specific activator of AurA.
  • Cep192/Spd-2 targets AurA to centrosomes, promoting its activation through an oligomerization-dependent mechanism.
  • This activation involves extensive T-loop phosphorylation, leading to high AurA kinase activity essential for microtubule organization.

Conclusions:

  • Cep192/Spd-2 is a critical regulator of AurA at centrosomes, essential for microtubule nucleation.
  • The findings reveal a novel oligomerization-dependent activation mechanism for AurA, distinct from previously known pathways.
  • This mechanism is conserved at other microtubule nucleation sites, such as kinetochores for Aurora B kinase activation.