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Infrared spectroscopy with multivariate analysis to interrogate endometrial tissue: a novel and objective diagnostic
S E Taylor1, K T Cheung, I I Patel
1Centre for Biophotonics, Lancaster Environment Centre, Lancaster University, Bailrigg, Lancaster LA1 4YQ, UK. siantaylor@gmail.com
British Journal of Cancer
|February 18, 2011
Summary
Infrared (IR) spectroscopy can differentiate between benign and malignant endometrial tissue. This objective test using attenuated total reflection Fourier-transform IR (ATR-FTIR) spectroscopy could enable earlier diagnosis of endometrial cancer.
Area of Science:
- Biomedical Engineering
- Spectroscopy
- Oncology
Background:
- Endometrial cancer is a common UK gynaecological malignancy.
- Current diagnosis relies on subjective microscopic interpretation of processed tissue.
- Infrared (IR) spectroscopy shows potential for distinguishing benign from malignant cells.
Purpose of the Study:
- To investigate the utility of ATR-FTIR spectroscopy for objective endometrial cancer diagnosis.
- To differentiate between benign and malignant endometrial tissue using spectral data.
Main Methods:
- Attenuated total reflection Fourier-transform IR (ATR-FTIR) spectroscopy was used on endometrial tissue samples from 76 patients.
- Principal component analysis and linear discriminant analysis were applied to spectral data.
- Histological verification was performed using haematoxylin and eosin staining.
Main Results:
- ATR-FTIR spectroscopy successfully distinguished between benign and malignant endometrial tissue.
- Spectral analysis highlighted the significance of lipid and protein structures (Amide I and II) in differentiation.
- Various subtypes of both benign and malignant tissues could be differentiated.
Conclusions:
- ATR-FTIR spectroscopy offers a potential for a simple, objective diagnostic test for endometrial cancer.
- This method could lead to earlier diagnosis, reducing disease morbidity and mortality.
