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RNA Splicing

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...

RNA Splicing

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...

The Spindle Assembly Checkpoint

The Spindle Assembly Checkpoint

The spindle assembly checkpoint is a molecular surveillance mechanism ensuring the fidelity of chromosome segregation during anaphase. The checkpoint monitors the completion of all the prerequisite steps before chromosome segregation to determine whether the segregation process should proceed or be delayed.
Many proteins function together to control the spindle assembly checkpoint. Mutations affecting these proteins may allow cells to proceed into anaphase prematurely, resulting in the...

The Spindle Assembly Checkpoint

The Spindle Assembly Checkpoint

The spindle assembly checkpoint is a molecular surveillance mechanism ensuring the fidelity of chromosome segregation during anaphase. The checkpoint monitors the completion of all the prerequisite steps before chromosome segregation to determine whether the segregation process should proceed or be delayed.
Many proteins function together to control the spindle assembly checkpoint. Mutations affecting these proteins may allow cells to proceed into anaphase prematurely, resulting in the...

The Mitotic Spindle

The Mitotic Spindle

The mitotic spindle—or spindle apparatus—is a eukaryotic, cytoskeletal structure made up of long protein fibers called microtubules. Formed during cell division, the spindle separates sister chromatids and moves them to opposite ends of a parental cell, where the now individual chromosomes are distributed to two daughter cell nuclei.
The bipolar configuration of the mitotic spindle facilitates chromosomal segregation, preparing the cell for division. One mechanism that ensures bipolar mitotic...

The Mitotic Spindle

The Mitotic Spindle

The mitotic spindle—or spindle apparatus—is a eukaryotic, cytoskeletal structure made up of long protein fibers called microtubules. Formed during cell division, the spindle separates sister chromatids and moves them to opposite ends of a parental cell, where the now individual chromosomes are distributed to two daughter cell nuclei.
The bipolar configuration of the mitotic spindle facilitates chromosomal segregation, preparing the cell for division. One mechanism that ensures bipolar mitotic...

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Related Experiment Video

Updated: Jun 4, 2026

Meiotic Spindle Assessment in Mouse Oocytes by siRNA-mediated Silencing

Meiotic Spindle Assessment in Mouse Oocytes by siRNA-mediated Silencing

Published on: October 11, 2015

The function of spliceosome components in open mitosis.

Jennifer C Hofmann¹, Alma Husedzinovic, Oliver J Gruss

¹DKFZ-ZMBH-Alliance, ZMBH, im Neuenheimer Feld, Heidelberg, Germany.

Nucleus (Austin, Tex.)

|February 18, 2011

Summary

Splicing, essential for gene expression, is crucial for cell division. This review explores whether splicing defects impair cell division indirectly or if spliceosomal proteins directly impact mitosis.

Keywords:

RNAi cell cycle chromatin mRNA processing open mitosis spliceosome splicing

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Last Updated: Jun 4, 2026

Meiotic Spindle Assessment in Mouse Oocytes by siRNA-mediated Silencing

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ACT1-CUP1 Assays Determine the Substrate-Specific Sensitivities of Spliceosomal Mutants in Budding Yeast

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A Reporter Based Cellular Assay for Monitoring Splicing Efficiency

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency

Published on: September 15, 2021

Area of Science:

Molecular Biology
Cell Biology
Genetics

Background:

Eukaryotic cells separate transcription (nucleus) from translation (cytoplasm), enabling mRNA processing.
Splicing removes introns from pre-mRNAs, yielding mature mRNAs and contributing to proteome diversity.
Splicing is vital for eukaryotic cell proliferation and division; its disruption often causes defects.

Purpose of the Study:

To review evidence for indirect or direct roles of splicing proteins in open mitosis.
To distinguish between splicing defects causing mitotic issues versus spliceosomal proteins directly influencing cell division.

Main Methods:

Review of existing scientific literature.
Analysis of experimental data on splicing factors and cell division.
Comparison of indirect and direct functional hypotheses.

Main Results:

Indirect evidence supports both hypotheses regarding splicing's role in mitosis.
Compromised splicing in interphase may indirectly affect mitosis.
Direct roles of spliceosomal proteins in open mitosis are also suggested by data.

Conclusions:

The precise role of splicing proteins in open mitosis remains to be fully elucidated.
Further experiments are needed to definitively distinguish between indirect and direct contributions.
Understanding these roles is critical for comprehending cell division regulation.