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Updated: Jun 4, 2026

Visualizing and Tracking Endogenous mRNAs in Live Drosophila melanogaster Egg Chambers
Published on: June 4, 2019
On the right track: following the nucleo-cytoplasmic path of an mRNA
Amir Mor1, Rakefet Ben-Yishay, Yaron Shav-Tal
1The Mina & Everard Goodman Faculty of Life Sciences & Institute of Nanotechnology, Bar-Ilan University, Ramat Gan, Israel.
Abstract:
The transcription machinery in the eukaryotic nucleus generates messenger RNA molecules that translocate through the nucleoplasm, anchor to a nuclear pore, and find their way out into the cytoplasm. The dynamic aspects of these steps in the expression pathway were examined in order to understand the kinetic time-frames of gene activation and message dissemination. Utilizing live-cell imaging and tracking of single mRNPs containing different sized mRNAs and varying numbers of introns and exons, it was possible to quantify the temporal and spatial characteristics of the nucleoplasmic travels of mRNPs as well as the kinetics of translocation through the nuclear pore.
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