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Related Experiment Video

Updated: Jun 4, 2026

Proteomic Profile of EPS-Urine through FASP Digestion and Data-Independent Analysis
14:48

Proteomic Profile of EPS-Urine through FASP Digestion and Data-Independent Analysis

Published on: May 8, 2021

Toward a standardized urine proteome analysis methodology.

Magali Court1, Nathalie Selevsek, Mariette Matondo

  • 1DECanBio consortium, France.

Proteomics
|February 18, 2011
PubMed
Summary
This summary is machine-generated.

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Standardized urine proteomics protocols were developed for reliable disease biomarker discovery. Organic solvent precipitation and in-solution digestion proved most effective for consistent, large-scale urinary proteome analysis.

Area of Science:

  • Biochemistry
  • Clinical Chemistry
  • Proteomics

Background:

  • Urine is a valuable source for routine clinical analysis of disease biomarkers.
  • The complexity of the urinary proteome presents challenges for large-scale studies, including sample collection and preparation.
  • Standardized analytical protocols are crucial for robust biomarker development pipelines.

Purpose of the Study:

  • To establish standardized and integrated analytical protocols for urine proteomics.
  • To evaluate and compare different sample processing methods for urinary biomarker discovery and verification.
  • To enable reproducible and accurate quantification of urinary proteins.

Main Methods:

  • Utilized two Liquid Chromatography-Mass Spectrometry (LC-MS) platforms: Accurate Mass and Time Tags (ấu) for discovery and Selected Reaction Monitoring (SRM) for verification.

More Related Videos

A Strategy for Sensitive, Large Scale Quantitative Metabolomics
14:18

A Strategy for Sensitive, Large Scale Quantitative Metabolomics

Published on: May 27, 2014

Related Experiment Videos

Last Updated: Jun 4, 2026

Proteomic Profile of EPS-Urine through FASP Digestion and Data-Independent Analysis
14:48

Proteomic Profile of EPS-Urine through FASP Digestion and Data-Independent Analysis

Published on: May 8, 2021

A Strategy for Sensitive, Large Scale Quantitative Metabolomics
14:18

A Strategy for Sensitive, Large Scale Quantitative Metabolomics

Published on: May 27, 2014

  • Processed urine samples from hospital patients using four distinct protocols.
  • Incorporated internal standards for estimating protein extraction yields and absolute protein quantification.

    • Main Results:

    • Organic solvent precipitation followed by in-solution digestion demonstrated superior performance compared to other evaluated methods.
    • This selected method was standardized for both discovery and verification phases.
    • Cross-validation between analytical platforms showed excellent consistency in urinary protein concentration estimates.

    Conclusions:

    • A standardized protocol involving organic solvent precipitation and in-solution digestion is effective for urine proteomics.
    • The developed protocol ensures reproducibility and accuracy for large-scale biomarker studies.
    • The findings support the use of standardized urine proteomics for reliable disease biomarker identification and validation.