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Indicators

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Certain organic substances change color in dilute solution when the hydronium ion concentration reaches a particular value. For example, phenolphthalein is a colorless substance in any aqueous solution with a hydronium ion concentration greater than 5.0 × 10−9 M (pH < 8.3). In more basic solutions where the hydronium ion concentration is less than 5.0 × 10−9 M (pH > 8.3), it is red or pink. Substances such as phenolphthalein, which can be used to determine the pH of a solution, are called...

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Rapid Colorimetric Assays to Qualitatively Distinguish RNA and DNA in Biomolecular Samples

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A colorimetric pH indicators and boronic acids ensemble array for quantitative sugar analysis.

Krishna Kanta Ghosh¹, Eunice Yap, Hanjo Kim

¹Department of Chemistry & MedChem Program of Life Sciences Institute, National University of Singapore, 117543, Singapore.

Chemical Communications (Cambridge, England)

|February 19, 2011

Summary

This study introduces a novel ensemble array using pH indicators and boronic acids for precise quantitative analysis of simple sugars. The method accurately measures mono- and disaccharides, demonstrating its utility in analyzing sugar content in saline solutions.

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Area of Science:

Analytical Chemistry
Biochemistry

Background:

Accurate quantification of carbohydrates is crucial in various scientific and clinical applications.
Existing methods for sugar analysis can be complex or require specialized equipment.

Purpose of the Study:

To develop and validate a novel ensemble array for the quantitative analysis of mono- and disaccharides.
To demonstrate the array's applicability in determining sugar content in clinically relevant samples.

Main Methods:

Utilized a colorimetric ensemble array composed of pH indicators and boronic acids.
Analyzed serial concentrations of various mono- and disaccharides.
Applied the array to quantify sugar levels in saline solutions.

Main Results:

The ensemble array exhibited distinct colorimetric response patterns correlating with specific sugar concentrations.
Achieved quantitative analysis of mono- and disaccharides with high accuracy.
Successfully quantified sugar content in clinically used saline solutions.

Conclusions:

The developed boronic acid and pH indicator ensemble array offers a robust and quantitative method for carbohydrate analysis.
This approach is suitable for determining sugar levels in complex matrices like saline solutions, with potential for broader clinical diagnostics.