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Updated: Jun 4, 2026

NMR-Based Fragment Screening in a Minimum Sample but Maximum Automation Mode
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Selective drug trace detection with low-field NMR.

Stefan Glöggler1, Meike Emondts, Johannes Colell

  • 1Institute of Technical and Macromolecular Chemistry, RWTH Aachen University, Worringer Weg 1, 52074 Aachen, Germany. stgloeggler@gmx.de

The Analyst
|February 19, 2011
PubMed
Summary

Para-hydrogen induced polarization (PHIP) enables sensitive detection of drugs at micromolar levels using portable low-field NMR. This technique can trace substances like nicotine, harmine, and morphine, even in complex solutions.

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Area of Science:

  • Nuclear Magnetic Resonance Spectroscopy
  • Chemical Biology
  • Analytical Chemistry

Background:

  • Portable low-field NMR offers new possibilities for sensitive chemical analysis.
  • Para-hydrogen induced polarization (PHIP) significantly enhances NMR signal intensity.

Purpose of the Study:

  • To investigate the potential of PHIP for sensitive drug detection using portable low-field NMR.
  • To demonstrate the capability of tracing drugs at micromolar concentrations and in challenging sample matrices.

Main Methods:

  • Application of para-hydrogen induced polarization (PHIP) for hyperpolarization.
  • Utilizing single-scan (1)H-NMR experiments on a portable low-field NMR system.
  • Selective enhancement of drug signals in proton-rich solutions.

Main Results:

  • Detection of nicotine at levels comparable to one cigarette.
  • Quantification of less than 1 mg of harmine and morphine.
  • Successful selective detection of drug signals masked by abundant protons.

Conclusions:

  • PHIP-enhanced portable low-field NMR is a powerful tool for sensitive drug tracing.
  • The technique is applicable to various drugs, including those relevant to pharmaceuticals and illicit substances.
  • PHIP overcomes signal suppression in complex mixtures, broadening NMR applications in drug analysis.