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Related Experiment Video

Updated: Jun 4, 2026

An ELISA Based Binding and Competition Method to Rapidly Determine Ligand-receptor Interactions

An ELISA Based Binding and Competition Method to Rapidly Determine Ligand-receptor Interactions

Published on: March 14, 2016

Radioligand binding assay.

G Thibault¹, E L Schiffrin

¹Laboratory of Cell Biology of Hypertension, Clinical Research Institute of Montréal, Montréal, Québec, C.

Methods in Molecular Medicine

|February 19, 2011

Summary

This summary is machine-generated.

Radioligand binding assays are crucial for characterizing angiotensin receptors (AT1 and AT2). This method quantifies receptor density and affinity using radiolabeled ligands and filtration or centrifugation.

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Published on: March 19, 2021

Area of Science:

Pharmacology
Biochemistry
Molecular Biology

Background:

Characterization and cloning of angiotensin receptors (AT1 and AT2) rely on assays measuring receptor density and affinity.
The radioligand binding assay is the primary methodological approach for studying these receptors.

Purpose of the Study:

To detail the test-tube radioligand binding assay for characterizing AT1 and AT2 receptors.
To explain how this assay measures receptor density and affinity.

Main Methods:

Utilizes radiolabeled ligands (e.g., (125)I or (3)H) incubated with membrane preparations or cultured cells.
Involves incubation with subtype-specific antagonists or agonists to reach equilibrium.
Separates bound tracer from free tracer via rapid filtration or centrifugation.

Main Results:

Quantifies bound tracer (cpm) to calculate receptor density and affinity.
Enables derivation of association/dissociation kinetics and saturation experiments.

Conclusions:

The radioligand binding assay is indispensable for the quantitative analysis of AT1 and AT2 receptors.
This method provides essential data for receptor characterization and drug development.