Related Experiment Video
Updated: Jun 4, 2026

06:01
Optimized PCR-based Detection of Mycoplasma
Published on: June 20, 2011
Routine quality control testing of cell cultures : detection of Mycoplasma
Methods in Molecular Medicine
|February 19, 2011
Abstract:
The quality of cell cultures and their products is important in scientific research and is also critical where they are used for diagnosis and therapies. Fundamental aspects of quality of cell cultures are purity, correct identity, and freedom from adventitious agents as well as appropriate biological characterization (1,2). Addressing these issues will ensure that experimental data and cell products meet the minimum requirements for scientific accuracy and regulatory approval.
Related Concept Videos
Methods to Assess Microbial Populations
Assessing microbial populations is crucial for understanding microbial roles in health, ecology, and industry. Various complementary techniques—both culture-based and molecular—enable detailed analysis of microbial abundance, diversity, and function.Viable Plate CountThe viable plate count is a traditional culture-based method used to estimate the number of living microbes in a sample. After serial dilution, the sample is spread onto nutrient agar plates. Each viable cell forms a visible...
Microbial Growth Measurement: Direct Methods
Direct methods for measuring microbial populations in a culture are essential tools in microbiology, providing quantitative data for various applications. Among these, microscopic counts, plate counts, and serial dilution are widely used techniques, each with unique principles and applications.Microscopic CountsMicroscopic counting involves the use of a Petroff-Hausser chamber, a specialized microscope slide with a grid and defined depth. By observing a liquid culture under a microscope,...
