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Determination of Viral Infectivity : HSV, VZV, and CMV
1Department of Virology, St. Bartholomew's and the Royal London School of Medicine and Dentistry, London, UK.
Methods in Molecular Medicine
|February 19, 2011
Summary
Developing antiviral drugs for herpesviruses is crucial due to limited options. This chapter details plaque reduction assays for herpesvirus antiviral drug development, excluding non-adherent viruses like Epstein-Barr virus.
Area of Science:
- Virology
- Antiviral Drug Development
Background:
- Limited availability of antiviral drugs compared to antibiotics.
- Herpesviruses are a significant focus for antiviral drug discovery.
- Various antiviral drugs targeting herpesviruses are in development.
Purpose of the Study:
- To describe simple plaque reduction assay methods for herpesviruses.
- To facilitate the evaluation of antiviral drug efficacy against herpesviruses.
Main Methods:
- Plaque reduction assays for herpesviruses grown in adherent cells.
- Exclusion of herpesviruses that do not grow in adherent cells (e.g., Epstein-Barr virus).
Main Results:
- The chapter provides accessible methods for herpesvirus plaque reduction assays.
- These methods are applicable to a range of herpesviruses requiring adherent cell cultures.
Conclusions:
- Plaque reduction assays are valuable tools for herpesvirus antiviral drug screening.
- Alternative methods are necessary for herpesviruses that do not grow in adherent cells.
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