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Related Concept Videos

Real Time RT-PCR02:57

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...

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Related Experiment Video

Updated: Jun 4, 2026

Determining 3'-Termini and Sequences of Nascent Single-Stranded Viral DNA Molecules during HIV-1 Reverse Transcription in Infected Cells
13:07

Determining 3'-Termini and Sequences of Nascent Single-Stranded Viral DNA Molecules during HIV-1 Reverse Transcription in Infected Cells

Published on: January 30, 2019

Line Probe Assay for Detecting Mutations in HIV-1 Reverse Transcriptase.

L Stuyver, A Wyseur, G Verpooten

    Methods in Molecular Medicine
    |February 19, 2011
    PubMed
    Summary
    This summary is machine-generated.

    Human immunodeficiency virus type 1 (HIV-1) replication is error-prone, leading to drug-resistant variants. Detecting these resistant HIV-1 quasispecies is crucial for effective antiretroviral treatment outcomes.

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    Rapid Screening of HIV Reverse Transcriptase and Integrase Inhibitors
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    Detection of Human Immunodeficiency Virus Type 1 (HIV-1) Antisense Protein (ASP) RNA Transcripts in Patients by Strand-Specific RT-PCR
    08:01

    Detection of Human Immunodeficiency Virus Type 1 (HIV-1) Antisense Protein (ASP) RNA Transcripts in Patients by Strand-Specific RT-PCR

    Published on: November 27, 2019

    Related Experiment Videos

    Last Updated: Jun 4, 2026

    Determining 3'-Termini and Sequences of Nascent Single-Stranded Viral DNA Molecules during HIV-1 Reverse Transcription in Infected Cells
    13:07

    Determining 3'-Termini and Sequences of Nascent Single-Stranded Viral DNA Molecules during HIV-1 Reverse Transcription in Infected Cells

    Published on: January 30, 2019

    Rapid Screening of HIV Reverse Transcriptase and Integrase Inhibitors
    05:46

    Rapid Screening of HIV Reverse Transcriptase and Integrase Inhibitors

    Published on: April 9, 2014

    Detection of Human Immunodeficiency Virus Type 1 (HIV-1) Antisense Protein (ASP) RNA Transcripts in Patients by Strand-Specific RT-PCR
    08:01

    Detection of Human Immunodeficiency Virus Type 1 (HIV-1) Antisense Protein (ASP) RNA Transcripts in Patients by Strand-Specific RT-PCR

    Published on: November 27, 2019

    Area of Science:

    • Virology
    • Molecular Biology
    • Immunology

    Background:

    • Human immunodeficiency virus type 1 (HIV-1) is a retrovirus known for its error-prone replication due to reverse transcriptase (RT).
    • This error-prone replication results in a quasi-species nature of the infection, with a high degree of genetic diversity within the host.
    • Antiretroviral therapies exert selective pressure, favoring the emergence and dominance of drug-resistant HIV-1 variants.

    Purpose of the Study:

    • To highlight the clinical significance of detecting drug-resistant HIV-1 quasispecies.
    • To underscore the impact of resistant variants on the efficacy of antiretroviral treatment.

    Main Methods:

    • The abstract does not specify methods but implies the study of HIV-1 replication and drug resistance.
    • Focuses on the selection of resistant variants under antiretroviral pressure (e.g., zidovudine, lamivudine).

    Main Results:

    • Antiretroviral drugs like AZT, ddC, ddI, d4T, and 3TC select for HIV-1 quasispecies resistant to these medications.
    • The emergence of these resistant variants is a direct consequence of the virus's error-prone replication.

    Conclusions:

    • Detection of drug-resistant HIV-1 variants is clinically important.
    • Identifying these variants can inform treatment strategies and potentially improve patient outcomes.