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Generation of a Mouse Spontaneous Autoimmune Thyroiditis Model
Published on: March 17, 2023
Experimental autoimmune thyroiditis in human parvovirus B19 transgenic mice.
Kouki Mori1, Katsumi Yoshida, Keiko Ishii
1Division of Nephrology, Endocrinology and Vascular Medicine, Tohoku University Graduate School of Medicine, Sendai, Japan. kokimori@yahoo.co.jp
Autoimmunity
|February 22, 2011
Summary
Human parvovirus B19 (B19) nonstructural protein 1 (NS1) gene introduction did not induce autoimmune thyroiditis in mice. Further research is needed to determine if B19 infection contributes to Hashimoto
Area of Science:
- Immunology
- Virology
- Endocrinology
Background:
- Autoimmune diseases are linked to viral infections.
- The role of human parvovirus B19 (B19) in Hashimoto's thyroiditis (HT) is under investigation.
- Previous studies suggest a potential association between B19 infection and HT.
Purpose of the Study:
- To investigate the potential role of B19 nonstructural protein 1 (NS1) in the development of autoimmune thyroiditis.
- To examine if the B19 NS1 gene can induce thyroiditis in a nonpermissive genetic background.
Main Methods:
- Transgenic C57BL/6 mice expressing the B19 NS1 gene were developed.
- Mice were immunized with thyroglobulin (Tg) or saline.
- Thyroiditis incidence, intrathyroidal T-cell receptor gene expression, and anti-Tg antibody levels were assessed.
Main Results:
- Thyroiditis was not observed in saline-treated mice or Tg-immunized males.
- Tg immunization induced thyroiditis in 25% of transgenic females, but not wild-type females.
- No significant differences in thyroiditis incidence, gene expression, or antibody levels were found between transgenic and wild-type females.
Conclusions:
- The B19 NS1 gene alone is insufficient to induce autoimmune thyroiditis in this mouse model.
- Further studies are necessary to definitively exclude the involvement of B19 infection in HT pathogenesis.
