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Applicability of RNA standards for evaluating RT-qPCR assays and platforms

Alison S Devonshire1, Ramnath Elaswarapu, Carole A Foy

  • 1LGC Limited, Queens Road, Teddington, Middlesex, TW11 0LY, UK.

BMC Genomics
|February 22, 2011
PubMed

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Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...