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Related Concept Videos

RNA Stability01:53

RNA Stability

Intact DNA strands can be found in fossils, while scientists sometimes struggle to keep RNA intact under laboratory conditions. The structural variations between RNA and DNA underlie the differences in their stability and longevity. Because DNA is double-stranded, it is inherently more stable. The single-stranded structure of RNA is less stable but also more flexible and can form weak internal bonds. Additionally, most RNAs in the cell are relatively short, while DNA can be up to 250 million...
RNA Stability01:53

RNA Stability

Intact DNA strands can be found in fossils, while scientists sometimes struggle to keep RNA intact under laboratory conditions. The structural variations between RNA and DNA underlie the differences in their stability and longevity. Because DNA is double-stranded, it is inherently more stable. The single-stranded structure of RNA is less stable but also more flexible and can form weak internal bonds. Additionally, most RNAs in the cell are relatively short, while DNA can be up to 250 million...
Types of RNA01:23

Types of RNA

Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
Types of RNA01:20

Types of RNA

Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in regulating gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA Performs Diverse...
Translational Regulation01:29

Translational Regulation

Translational regulation in prokaryotes ensures efficient protein synthesis by controlling ribosome access to mRNA. This regulation is mediated by secondary RNA structures, including translational riboswitches, RNA thermometers, and small RNAs (sRNAs), which respond to intracellular and environmental signals to modulate gene expression.Translational RiboswitchesRiboswitches in the leader region of mRNAs can regulate translation by altering the accessibility of the Shine-Dalgarno (SD) sequence,...
mRNA Stability and Gene Expression02:51

mRNA Stability and Gene Expression

The structure and stability of mRNA molecules regulates gene expression, as mRNAs are a key step in the pathway from gene to protein. In eukaryotes, the half-life of mRNA varies from a few minutes up to several days. mRNA stability is essential in growth and development. The absence of the proteins regulating its stability, such as tristetraprolin in mice, can cause systemic issues, including bone marrow overgrowth, inflammation, and autoimmunity.
Cis-acting Elements involved in mRNA stability

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MS2-Affinity Purification Coupled with RNA Sequencing in Gram-Positive Bacteria
08:34

MS2-Affinity Purification Coupled with RNA Sequencing in Gram-Positive Bacteria

Published on: February 23, 2021

Regulated RNA stability in the Gram positives.

Ciarán Condon1, David H Bechhofer

  • 1CNRS UPR 9073 (affiliated with Université de Paris 7 - Denis Diderot), Institut de Biologie Physico-Chimique, 13 rue Pierre et Marie Curie, 75005 Paris, France.

Current Opinion in Microbiology
|February 22, 2011
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Summary

Regulated RNA decay controls bacterial gene expression in Gram-positive bacteria like Bacillus subtilis. New ribonuclease discoveries, including RNase J1, reveal novel post-transcriptional regulatory mechanisms.

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Area of Science:

  • Microbiology
  • Molecular Biology
  • Genetics

Background:

  • Post-transcriptional regulation is a key bacterial gene expression control mechanism.
  • RNA decay pathways play a significant role in this regulation.
  • Mechanisms of RNA decay are less understood compared to transcriptional control.

Purpose of the Study:

  • To highlight the importance of regulated RNA decay in Gram-positive bacteria.
  • To focus on Bacillus subtilis as a model organism.
  • To discuss novel ribonuclease activities and their regulatory roles.

Main Methods:

  • Review of existing literature on bacterial RNA decay.
  • Focus on specific ribonuclease discoveries in Bacillus subtilis.
  • Analysis of the dual function of RNase J1 (endonuclease and 5'-to-3' exoribonuclease).

Main Results:

  • Identification of novel ribonuclease activities in Gram-positive bacteria.
  • Characterization of RNase J1 with dual endonuclease and exoribonuclease functions.
  • Recognition of RNA decay as a significant post-transcriptional regulatory mechanism.

Conclusions:

  • Regulated RNA decay is a crucial mechanism for controlling gene expression in Gram-positive bacteria.
  • RNase J1 represents a key enzyme in bacterial RNA metabolism and regulation.
  • Further research into RNA decay pathways will uncover more intricate gene regulation strategies.