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[Immobilization of microbial lactase]
A Illanes1, M E Zúñiga, A Ruiz
1Escuela de Ingeniería Bioquímica, Universidad Católica de Valparaíso, Chile.
Archivos De Biologia Y Medicina Experimentales
|October 1, 1990
Summary
This study developed an in situ immobilized fungal lactase enzyme catalyst for continuous cheese whey hydrolysis. The immobilized lactase demonstrated a 120-day operational half-life, offering a stable solution for dairy industry applications.
Area of Science:
- Biocatalysis
- Enzyme immobilization
- Dairy technology
Context:
- Lactose in dairy products presents challenges for the food industry.
- Cheese whey valorization is crucial for sustainable dairy processing.
- Enzyme catalysts are key for efficient lactose hydrolysis.
Purpose:
- To develop and scale-up an in situ immobilized fungal lactase system for continuous whey permeate hydrolysis.
- To evaluate the performance of packed bed reactors with immobilized lactase.
- To assess the operational stability of the immobilized enzyme catalyst.
Summary:
- Fungal lactase was immobilized in situ within a reactor on activated chitin for continuous whey permeate hydrolysis.
- Packed bed reactors showed deviations from theoretical models at high flowrates due to backmixing and channeling.
- The immobilized lactase achieved a productivity of 58 g glucose/l.h and demonstrated a 120-day operational half-life at 40°C and pH 4.0.
Impact:
- Provides a stable and efficient biocatalyst for lactose hydrolysis in the dairy industry.
- Enables the recovery and upgrading of cheese whey, reducing waste and creating value-added products.
- Optimizes continuous hydrolysis processes, improving substrate conversion and reactor productivity.