Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

[Immobilization of microbial lactase].

A Illanes1, M E Zúñiga, A Ruiz

  • 1Escuela de Ingeniería Bioquímica, Universidad Católica de Valparaíso, Chile.

Archivos De Biologia Y Medicina Experimentales
|October 1, 1990
PubMed
Summary

This study developed an in situ immobilized fungal lactase enzyme catalyst for continuous cheese whey hydrolysis. The immobilized lactase demonstrated a 120-day operational half-life, offering a stable solution for dairy industry applications.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Texture differentiation using audio signal analysis with robotic interventional instruments.

Computers in biology and medicine·2019
Same author

Passive artifact behavior prediction of interventional tools in high-field MRI using a 0.55T portable benchtop MR scanner.

Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual International Conference·2017
Same author

Solid substrate fermentation of leached beet pulp withTrichoderma aureoviride.

World journal of microbiology & biotechnology·2014
Same author

Measurement of the Bc+ meson lifetime using the decay mode Bc+ --> J/Psie+nue.

Physical review letters·2006
Same author

Association of genetic markers within the KIT and KITLG genes with human male infertility.

Human reproduction (Oxford, England)·2006
Same author

The "El Alamo" project (1990-1997): two consecutive hospital-based studies of breast cancer outcomes in Spain.

Clinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico·2006

Area of Science:

  • Biocatalysis
  • Enzyme immobilization
  • Dairy technology

Context:

  • Lactose in dairy products presents challenges for the food industry.
  • Cheese whey valorization is crucial for sustainable dairy processing.
  • Enzyme catalysts are key for efficient lactose hydrolysis.

Purpose:

  • To develop and scale-up an in situ immobilized fungal lactase system for continuous whey permeate hydrolysis.
  • To evaluate the performance of packed bed reactors with immobilized lactase.
  • To assess the operational stability of the immobilized enzyme catalyst.

Summary:

  • Fungal lactase was immobilized in situ within a reactor on activated chitin for continuous whey permeate hydrolysis.
  • Packed bed reactors showed deviations from theoretical models at high flowrates due to backmixing and channeling.
  • The immobilized lactase achieved a productivity of 58 g glucose/l.h and demonstrated a 120-day operational half-life at 40°C and pH 4.0.

Impact:

  • Provides a stable and efficient biocatalyst for lactose hydrolysis in the dairy industry.
  • Enables the recovery and upgrading of cheese whey, reducing waste and creating value-added products.
  • Optimizes continuous hydrolysis processes, improving substrate conversion and reactor productivity.

Related Experiment Videos