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Updated: Aug 6, 2026
11:15
In Situ Detection of Bacteria within Paraffin-embedded Tissues Using a Digoxin-labeled DNA Probe Targeting 16S rRNA
Published on: May 21, 2015
[Microbiological study in clinically characterized rapidly progressive periodontal disease]
H Masunaga1, M Matsue, I Matsue
1Department of Periodontology, Nihon University School of Dentistry at Matsudo.
Nihon Shishubyo Gakkai Kaishi
|March 1, 1990
Summary
Periodontal disease treatment success varies, with some patients showing improvement. However, even effective treatments leave subgingival bacterial profiles distinct from healthy gums, indicating persistent microbial shifts in periodontal pockets.
Area of Science:
- Periodontology
- Microbiology
- Dental Therapeutics
Background:
- Rapidly progressive periodontal disease (RPPD) presents complex challenges in treatment and microbial management.
- Understanding the subgingival microflora is crucial for assessing periodontal health and treatment efficacy.
- Specific bacterial species, such as Bacteroides, are implicated in periodontal disease pathogenesis.
Purpose of the Study:
- To analyze the predominant cultivable subgingival microflora in patients with RPPD before and after periodontal treatment.
- To correlate specific microbial profiles with treatment outcomes, distinguishing between effective and non-effective sites.
- To investigate the potential of microbial analysis for indicating active or progressing periodontitis.
Main Methods:
- Subgingival bacterial samples were collected from two sites (≥6 mm pockets) in ten RPPD patients.
- Microflora analysis was performed at pre-treatment and post-treatment stages (three weeks after root curettage).
- Microbial composition was compared between effective sites, non-effective sites, and healthy gingival controls.
Main Results:
- Pre-treatment diseased sites showed increased proportions of Bacteroides sp., particularly B. intermedius and B. gingivalis.
- Post-treatment, B. intermedius and B. gingivalis decreased, but effective sites still differed significantly from healthy sites.
- Non-effective sites were associated with higher proportions of B. intermedius and E. corrodens; H. actinomycetemcomitans was consistently detected.
Conclusions:
- Periodontal treatment can reduce key pathogenic bacteria like B. gingivalis, but subgingival flora remains altered post-treatment.
- Specific microbial signatures, including elevated B. intermedius and E. corrodens, may indicate non-effective treatment outcomes.
- Pre-treatment microbial analysis can help identify progressing periodontitis, suggesting that host resistance may also play a role.
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