Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Sputum Studies II: Culture and Sensitivity01:20

Sputum Studies II: Culture and Sensitivity

Description
Sputum culture and sensitivity is a medical procedure used to diagnose bacterial infections in the respiratory tract and select the most appropriate antibiotics for treatment. This process involves analyzing sputum samples of thick and opaque secretions produced in the lungs and airways. These samples are collected from patients and then sent to the laboratory for analysis.
The test can identify various pathogens responsible for respiratory infections, including Streptococcus,...
Sputum Studies I: Gram Stain, cytology, and Acid-fast smear and culture01:26

Sputum Studies I: Gram Stain, cytology, and Acid-fast smear and culture

Sputum studies are a critical part of diagnosing and treating numerous respiratory conditions. These studies involve obtaining sputum samples for analysis to identify pathogenic organisms and assess the presence of abnormal cells indicative of malignant conditions. This lesson will delve into three fundamental sputum studies: Gram Stain, Cytology, and Acid-fast Smear and Culture.
Gram Stain
The Gram Stain is an integral part of sputum studies. It involves the staining of sputum, which permits...
Sampling Methods: Sample Types01:18

Sampling Methods: Sample Types

Sampling materials are classified into three main types: solid, liquid, and gas.
Solid samples include a variety of substances, such as sediments from water bodies, soil, metals, and biological tissues. Two standard methods for extracting sediments from water bodies are grab sampling and piston coring. Grab sampling involves using a device to collect a discrete sediment sample from the bottom of a water body with minimal disturbance. Grab samples do not always represent the entire area due to...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Corrigendum to "Does current reporting of lung function by the UK cystic fibrosis registry allow a fair comparison of adult centres?" [J Cyst Fibros (2017) 585-591].

Journal of cystic fibrosis : official journal of the European Cystic Fibrosis Society·2017
Same author

Do selective topical beta antagonists for glaucoma have respiratory side effects?

The British journal of ophthalmology·2004
Same author

Glucocorticoid treatment reduces exhaled nitric oxide in cystic fibrosis patients.

The European respiratory journal·2001
Same author

A polymorphism in the tumor necrosis factor-alpha gene promoter region may predispose to a poor prognosis in COPD.

Chest·2000
Same author

Airway inflammation after controlled exposure to diesel exhaust particulates.

American journal of respiratory and critical care medicine·2000
Same author

No effect of inhaled budesonide on the response to inhaled ozone in normal subjects.

American journal of respiratory and critical care medicine·2000

Related Experiment Video

Updated: Jun 4, 2026

Methodology for Sputum Induction and Laboratory Processing
13:28

Methodology for Sputum Induction and Laboratory Processing

Published on: December 17, 2017

Induced sputum : whole sample.

V M Keatings1, J A Nightingale

  • 1Department of Medicine, Education and Research Centre, St. Vincent's Hospital, Elm Park, Dublin, Ireland.

Methods in Molecular Medicine
|February 22, 2011
PubMed
Summary
This summary is machine-generated.

Broncho-alveolar lavage (BAL) via bronchoscopy aids airway inflammation research in asthma and COPD. However, its invasive nature limits studies to mild cases, necessitating extrapolation and hindering kinetic analysis.

More Related Videos

Capturing Actively Produced Microbial Volatile Organic Compounds from Human-Associated Samples with Vacuum-Assisted Sorbent Extraction
09:19

Capturing Actively Produced Microbial Volatile Organic Compounds from Human-Associated Samples with Vacuum-Assisted Sorbent Extraction

Published on: June 1, 2022

Quality-Controlled Sputum Analysis by Flow Cytometry
07:22

Quality-Controlled Sputum Analysis by Flow Cytometry

Published on: August 9, 2021

Related Experiment Videos

Last Updated: Jun 4, 2026

Methodology for Sputum Induction and Laboratory Processing
13:28

Methodology for Sputum Induction and Laboratory Processing

Published on: December 17, 2017

Capturing Actively Produced Microbial Volatile Organic Compounds from Human-Associated Samples with Vacuum-Assisted Sorbent Extraction
09:19

Capturing Actively Produced Microbial Volatile Organic Compounds from Human-Associated Samples with Vacuum-Assisted Sorbent Extraction

Published on: June 1, 2022

Quality-Controlled Sputum Analysis by Flow Cytometry
07:22

Quality-Controlled Sputum Analysis by Flow Cytometry

Published on: August 9, 2021

Area of Science:

  • Pulmonary Medicine
  • Respiratory Research
  • Inflammation Studies

Background:

  • Fiberoptic bronchoscopy enables airway inflammation characterization in asthma and COPD.
  • Broncho-alveolar lavage (BAL) and biopsy are key diagnostic tools.
  • Inflammation is recognized as central to asthma and COPD pathogenesis.

Purpose of the Study:

  • To evaluate the utility and limitations of bronchoscopy-based research methods.
  • To understand the role of inflammation in airways diseases.
  • To address challenges in studying airway inflammation kinetics.

Main Methods:

  • Bronchoscopic biopsy and broncho-alveolar lavage (BAL) in patients and controls.
  • Characterization of airway inflammation.
  • Ethical considerations for research involving invasive procedures.

Main Results:

  • BAL allows inflammation assessment in mild airflow obstruction.
  • Invasive nature limits research to specific patient groups.
  • Procedure invasiveness restricts repeated sampling for kinetic studies.

Conclusions:

  • Bronchoscopy-derived data from mild cases require extrapolation to broader patient populations.
  • The invasive nature of bronchoscopy poses ethical and practical challenges for research.
  • Alternative or refined methods may be needed to study airway inflammation dynamics comprehensively.