Related Experiment Video
Updated: Jun 4, 2026
Application of Optical Coherence Tomography to a Mouse Model of Retinopathy
Published on: January 12, 2022
Interchangeability of macular thickness measurements between different volumetric protocols of Spectralis optical
Yaroslava Wenner1, Stephan Wismann, Melanie Jäger
1Department of Ophthalmology, Justus-Liebig-University Giessen, Universitätsklinikum Giessen & Marburg GmbH, Giessen Campus, Friedrichstr. 18, 35392 Giessen, Germany. yaroslava.chopovska@augen.med.uni-giessen.de
Backgrounds:
To evaluate in healthy eyes the interchangeability of retinal thickness measurements resulting from different protocols of Spectralis optical coherence tomography (OCT, Heidelberg Engineering, Germany).
Methods:
Three different central volumetric protocols were performed in one session by one single operator in a randomly selected eye of 31 healthy adults (range, 19-30 years): (1) area 6 × 6 mm, 25 scan pattern (SP), (2) area 6 × 6 mm, 49 SP, (3) area 6 × 3 mm, 97 SP. Nine ETDRS areas were analyzed, except outer areas for 97 SP. Interclass correlation coefficients (ICC) and limits of agreement (Bland-Altman) were calculated.
Results:
Mean minimal foveal thickness was 224.0 ± 15.8 μm and central retinal thickness 280.8 ± 16.5 μm. ICC ranged from 0.973 to 0.993. Ninety-five percent limits of agreement between the protocols were <7 μm in all ETDRS areas. The difference between two measurements never exceeded 9 μm.
Conclusions:
Spectralis OCT retinal thickness measurements in healthy subjects showed good protocol interchangeability for all tested protocols. Larger differences are to be expected in eyes with macular pathology.
