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Related Concept Videos

Flow Cytometry01:23

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Related Experiment Video

Updated: Jun 4, 2026

A Flow Cytometry-based Assay to Identify Compounds That Disrupt Binding of Fluorescently-labeled CXC Chemokine Ligand 12 to CXC Chemokine Receptor 4
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A Flow Cytometry-based Assay to Identify Compounds That Disrupt Binding of Fluorescently-labeled CXC Chemokine Ligand 12 to CXC Chemokine Receptor 4

Published on: March 10, 2018

Flow cytometric analysis.

P F McKay1

  • 1Department of Viral Pathogenesis, Beth Israel Deaconess Medical Center and Department of Medicine, Harvard Medical School, Boston, MA.

Methods in Molecular Medicine
|February 22, 2011
PubMed
Summary
This summary is machine-generated.

Flow cytometry analyzes fluorescently labeled cells in fluid flow. Its widespread use is driven by the increasing availability of specific antibodies for cell analysis.

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Last Updated: Jun 4, 2026

A Flow Cytometry-based Assay to Identify Compounds That Disrupt Binding of Fluorescently-labeled CXC Chemokine Ligand 12 to CXC Chemokine Receptor 4
06:56

A Flow Cytometry-based Assay to Identify Compounds That Disrupt Binding of Fluorescently-labeled CXC Chemokine Ligand 12 to CXC Chemokine Receptor 4

Published on: March 10, 2018

Techniques for the Analysis of Extracellular Vesicles Using Flow Cytometry
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Techniques for the Analysis of Extracellular Vesicles Using Flow Cytometry

Published on: March 17, 2015

Flow Cytometric Analysis of Bimolecular Fluorescence Complementation: A High Throughput Quantitative Method to Study Protein-protein Interaction
11:11

Flow Cytometric Analysis of Bimolecular Fluorescence Complementation: A High Throughput Quantitative Method to Study Protein-protein Interaction

Published on: August 15, 2013

Area of Science:

  • Immunology
  • Cell Biology
  • Biotechnology

Background:

  • Flow cytometry is a powerful technique for analyzing cells.
  • The technique relies on fluorescently labeled cells in a fluid stream.
  • Advancements in antibody technology have significantly boosted its utility.

Purpose of the Study:

  • To explain the principles of flow cytometry.
  • To highlight the factors contributing to its widespread adoption.
  • To underscore the importance of antibody availability in cell analysis.

Main Methods:

  • Analysis of cells in fluid flow.
  • Utilizing fluorescent labels on cells.
  • Employing specific monoclonal and polyclonal antibodies.

Main Results:

  • Flow cytometry enables detailed cell analysis.
  • Increased availability of specific antibodies drives technique adoption.
  • Commercially available antibodies facilitate diverse assay applications.

Conclusions:

  • Flow cytometry is a versatile and widely adopted cell analysis technique.
  • The expansion of specific antibody development is key to its success.
  • Ready access to labeled antibodies supports broad research applications.