Immunocytochemistry

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S V Noorden1

  • 1Department of Histochemistry, Division of Diagnostic Sciences, Imperial College School of Medicine, London, UK.

Methods in Molecular Medicine
|February 22, 2011
PubMed

Immunocytochemistry provides an accurate way of localizing antigens in situ in tissue sections or cell preparations using antibodies combined with a label that can be identified microscopically. The most frequently used labels are fluorescent compounds that can be excited by light of specific wavelength, enzymes that can act on a specific substrate with a chromogen to give a colored end-product, and colloidal gold (for electron microscopy). Antibodies are applied to tissue preparations, often in a series of layers, one applied antibody acting as an antigen for the next (antispecies) antibody to build up the amount of label at the original site of reaction.

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