Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

DNA Bacteriophages01:26

DNA Bacteriophages

Bacteriophages, or phages, are viruses that specifically infect bacteria, utilizing their genetic material to hijack host cellular machinery for replication. DNA bacteriophages employ single-stranded DNA (ssDNA) or double-stranded DNA (dsDNA) genomes. These phages exhibit diverse replication strategies and host interactions, influencing their ecological roles and applications in biotechnology and medicine.ssDNA BacteriophagesssDNA phages, with their small genomes, utilize unique strategies to...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A multicenter, randomized phase 2 study to establish combinations of CBP501, cisplatin and nivolumab for ≥3rd-line treatment of patients with advanced pancreatic adenocarcinoma.

European journal of cancer (Oxford, England : 1990)·2024
Same author

Does the association between substance use and sexual risk behaviors among high school students vary by sexual identity?

Addictive behaviors·2019
Same author

A low balance between microparticles expressing tissue factor pathway inhibitor and tissue factor is associated with thrombosis in Behçet's Syndrome.

Scientific reports·2016
Same author

Immune senescence: significance of the stromal microenvironment.

Clinical and experimental immunology·2016
Same author

The exaggerated inflammatory response in Behçet's syndrome: identification of dysfunctional post-transcriptional regulation of the IFN-γ/CXCL10 IP-10 pathway.

Clinical and experimental immunology·2015
Same author

Computational and in vivo investigation of optical reflectance from human brain to assist neurosurgery.

Journal of biomedical optics·2012
Same journal

Erratum to: Immunotherapeutic Approach to Cancer with Cutaneous DNA Vaccination.

Methods in molecular medicine·2015
Same journal

Methods for cancer gene therapy using tumor suppressor genes.

Methods in molecular medicine·2014
Same journal

Suppression of the human carcinoma phenotype by an antioncogene ribozyme.

Methods in molecular medicine·2014
Same journal

Methods for the use of stromal cells for therapeutic gene therapy.

Methods in molecular medicine·2014
Same journal

Methods for adenovirus-mediated gene transfer to synovium in vivo.

Methods in molecular medicine·2014
Same journal

Methods for gene transfer to synovium.

Methods in molecular medicine·2014
See all related articles

Related Experiment Video

Updated: Jun 4, 2026

A Protocol for Phage Display and Affinity Selection Using Recombinant Protein Baits
12:36

A Protocol for Phage Display and Affinity Selection Using Recombinant Protein Baits

Published on: February 16, 2014

Phage display technology : protocols.

M Johns1, D B Palmer

  • 1Department of Immunology, Imperial College School of Medicine, London, UK.

Methods in Molecular Medicine
|February 22, 2011
PubMed
Summary
This summary is machine-generated.

Genetic engineering enables antibody fragments to be displayed on filamentous phage. This technique allows for the isolation of antibody fragments, including single-chain variable fragments (scFv) and fragment antigen-binding (Fab) fragments, using phage display technology.

More Related Videos

Using Phage Display to Develop Ubiquitin Variant Modulators for E3 Ligases
06:30

Using Phage Display to Develop Ubiquitin Variant Modulators for E3 Ligases

Published on: August 27, 2021

Interactome-Seq: A Protocol for Domainome Library Construction, Validation and Selection by Phage Display and Next Generation Sequencing
12:04

Interactome-Seq: A Protocol for Domainome Library Construction, Validation and Selection by Phage Display and Next Generation Sequencing

Published on: October 3, 2018

Related Experiment Videos

Last Updated: Jun 4, 2026

A Protocol for Phage Display and Affinity Selection Using Recombinant Protein Baits
12:36

A Protocol for Phage Display and Affinity Selection Using Recombinant Protein Baits

Published on: February 16, 2014

Using Phage Display to Develop Ubiquitin Variant Modulators for E3 Ligases
06:30

Using Phage Display to Develop Ubiquitin Variant Modulators for E3 Ligases

Published on: August 27, 2021

Interactome-Seq: A Protocol for Domainome Library Construction, Validation and Selection by Phage Display and Next Generation Sequencing
12:04

Interactome-Seq: A Protocol for Domainome Library Construction, Validation and Selection by Phage Display and Next Generation Sequencing

Published on: October 3, 2018

Area of Science:

  • Biotechnology
  • Molecular Biology
  • Immunology

Background:

  • Significant advancements in genetic engineering have focused on antibody molecule manipulation.
  • Filamentous phage have emerged as a key platform for displaying engineered biomolecules.

Purpose of the Study:

  • To explore the utility of filamentous phage as a display system for antibody fragments.
  • To investigate the successful cloning and expression of antibody fragment libraries on phage surfaces.

Main Methods:

  • Utilized genetic engineering techniques to manipulate antibody molecules.
  • Employed filamentous phage display to express antibody fragments, including single-chain variable fragments (sFv) and fragment antigen-binding (Fab) fragments.
  • Investigated expression via the N-terminal gene III product and the gene VIII product of bacteriophages.

Main Results:

  • Successfully cloned and displayed diverse antibody fragments, such as sFv and Fab, on the surface of filamentous phage.
  • Demonstrated the feasibility of using phage display for isolating antibodies with varying affinities.
  • Expression was achieved using both gene III and gene VIII products of bacteriophages.

Conclusions:

  • Filamentous phage display is an effective strategy for engineering and presenting antibody fragments.
  • This technology facilitates the isolation of specific antibody fragments, including those with lower affinities.
  • Phage display represents a powerful tool in antibody engineering and discovery.