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Related Concept Videos

Immunofluorescence Microscopy01:12

Immunofluorescence Microscopy

A fluorescence microscope uses fluorescent chromophores called fluorochromes, which can absorb energy from a light source and then emit this energy as visible light. Fluorochromes include naturally fluorescent substances (such as chlorophylls) and fluorescent stains that are added to the specimen to create contrast. Dyes such as Texas red and FITC are examples of fluorochromes. Other examples include the nucleic acid dyes 4’,6’-diamidino-2-phenylindole (DAPI), and acridine orange.
The...
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.

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Related Experiment Video

Updated: Jun 4, 2026

Fabricating a UV-Vis and Raman Spectroscopy Immunoassay Platform
09:02

Fabricating a UV-Vis and Raman Spectroscopy Immunoassay Platform

Published on: November 10, 2016

Supercritical angle fluorescence immunoassay platform.

Thomas Ruckstuhl1, Christian M Winterflood, Stefan Seeger

  • 1Physikalisch-Chemisches Institut, Universität Zürich, Winterthurerstrasse 190, CH-8057 Zürich, Switzerland.

Analytical Chemistry
|February 23, 2011
PubMed
Summary
This summary is machine-generated.

A new, affordable immunoassay platform uses polymer test tubes and a fluorescence reader for rapid, sensitive analyte detection. This technology offers a faster, simpler alternative to traditional enzyme-linked immunosorbent assays (ELISA).

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Immunofluorescence Analysis of Endogenous and Exogenous Centromere-kinetochore Proteins
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Immunofluorescence Analysis of Endogenous and Exogenous Centromere-kinetochore Proteins

Published on: March 3, 2016

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Last Updated: Jun 4, 2026

Fabricating a UV-Vis and Raman Spectroscopy Immunoassay Platform
09:02

Fabricating a UV-Vis and Raman Spectroscopy Immunoassay Platform

Published on: November 10, 2016

Excitation-Scanning Hyperspectral Imaging Microscopy to Efficiently Discriminate Fluorescence Signals
07:34

Excitation-Scanning Hyperspectral Imaging Microscopy to Efficiently Discriminate Fluorescence Signals

Published on: August 22, 2019

Immunofluorescence Analysis of Endogenous and Exogenous Centromere-kinetochore Proteins
05:35

Immunofluorescence Analysis of Endogenous and Exogenous Centromere-kinetochore Proteins

Published on: March 3, 2016

Area of Science:

  • Biotechnology and Biomedical Engineering
  • Analytical Chemistry
  • Immunodiagnostics

Background:

  • Traditional enzyme-linked immunosorbent assays (ELISA) are widely used but are often time-consuming and labor-intensive.
  • There is a need for rapid, sensitive, and user-friendly platforms for analyte detection in various biological and chemical applications.

Purpose of the Study:

  • To develop an inexpensive, easy-to-use immunoassay platform for sensitive analyte detection.
  • To demonstrate the platform's capability for real-time, wash-free solid-phase immunoassays.
  • To evaluate the platform's performance using interleukin-2 (IL-2) detection.

Main Methods:

  • Development of single-use polymer test tubes with integrated optics for supercritical angle fluorescence (SAF) capture.
  • Immobilization of capture antibodies within the test tubes for one-step sandwich immunoassays.
  • Utilized a compact fluorescence reader for real-time signal detection without washing steps.

Main Results:

  • Achieved a limit of detection of 0.27 pM (4.5 pg/mL) for interleukin-2 (IL-2) within a 12-minute turnaround time.
  • Demonstrated a linear detection range spanning three orders of magnitude.
  • The developed technology is adaptable to well plates, indicating broad applicability.

Conclusions:

  • The developed SAF-based immunoassay platform offers a sensitive, rapid, and cost-effective alternative to conventional methods like ELISA.
  • The platform's ease of use and real-time, wash-free detection capabilities make it suitable for various diagnostic applications.
  • This technology holds significant potential for replacing labor-intensive and time-consuming immunoassays.