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Updated: Jun 4, 2026

Assessment of Resistance to Tyrosine Kinase Inhibitors by an Interrogation of Signal Transduction Pathways by Antibody Arrays

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Published on: September 19, 2018

Repeatedly identified differentially expressed proteins (RIDEPs) from antibody microarray proteomic analysis.

Victoria C Hodgkinson¹, Dalia ElFadl, Philip J Drew

¹Cancer Biology Proteomics Group, Postgraduate Medical Institute of the University of Hull, Hull, UK.

Journal of Proteomics

|February 23, 2011

Summary

Repeatedly identified differentially expressed proteins (RIDEPs) were identified using antibody microarrays in oncology research. This study reports 13 potential RIDEPs, aiding biomarker verification in proteomics.

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Assessment of Resistance to Tyrosine Kinase Inhibitors by an Interrogation of Signal Transduction Pathways by Antibody Arrays

Assessment of Resistance to Tyrosine Kinase Inhibitors by an Interrogation of Signal Transduction Pathways by Antibody Arrays

Published on: September 19, 2018

Extracellular Protein Microarray Technology for High Throughput Detection of Low Affinity Receptor-Ligand Interactions

Extracellular Protein Microarray Technology for High Throughput Detection of Low Affinity Receptor-Ligand Interactions

Published on: January 7, 2019

Area of Science:

Proteomics
Biomarker Discovery
Oncology Research

Background:

Antibody microarrays are key tools in comparative proteomics.
Reliable biomarker discovery depends on high-quality data and protein selection.
Previous meta-analyses identified repeatedly identified differentially expressed proteins (RIDEPs) in mass spectrometry studies.

Purpose of the Study:

To assess RIDEPs using antibody microarray data.
To identify potential RIDEPs from antibody microarray experiments.
To aid in the interpretation of experimental data and prioritize proteins for verification.

Main Methods:

Utilized a commercial 725 antibody microarray platform (Panorama XPRESS Profiler725).
Analyzed 13 independent experiments from 5 distinct human sample groups (tissue, cells, cell lines).
Defined significant fold changes as ≥1.8, with analysis performed by a single individual to minimize variability.

Main Results:

Identified 13 RIDEPs from the antibody microarray data.
Each RIDEP appeared in at least 4/13 (30%) of the analyses.
RIDEPs were found across at least 2 out of 5 experimental sample groups.

Conclusions:

The phenomenon of RIDEPs appears to exist in antibody microarray proteomics.
A preliminary list of 13 RIDEPs from the XPRESS Profiler725 platform is provided.
This list can assist in interpreting experimental data and prioritizing differentially expressed proteins for verification.