Developing custom chinese hamster ovary-host cell protein assays using acoustic membrane microparticle technology

Area of Science:

• Biotechnology
• Assay Development
• Biosensor Technology

Background:

• Traditional immunoassays like ELISA and Western blots are time-consuming and complex for custom protein detection.
• Development of novel platforms for sensitive and reproducible protein quantitation is crucial for bioprocess monitoring.

Purpose of the Study:

• To outline a protocol for developing custom immunoassays using BioScale's Acoustic Membrane MicroParticle (AMMP) technology.
• To demonstrate the application of AMMP technology for developing a Chinese Hamster Ovary-Host Cell Protein (CHO-HCP) assay for bioprocess development.

Main Methods:

• Utilized BioScale's proprietary AMMP technology and ViBE platform for sandwich immunoassay development.
• Employed a biosensor with functionalized sensor chips, antibody-labeled magnetic microparticles, and hapten-tagged antibodies.
• Measured antigen concentration by quantifying changes in oscillation frequency due to microparticle binding to the sensor surface.

Main Results:

• The AMMP assay demonstrated improved sensitivity and reproducibility compared to traditional methods.
• The protocol successfully facilitated the development of a custom CHO-HCP assay.
• The biosensor allowed for sequential analysis of samples, enabling efficient workflow.

Conclusions:

• AMMP technology offers a streamlined and efficient approach for developing custom immunoassays.
• This technology provides enhanced performance, reduced operator time, and automated operation for bioprocess development.
• The principles outlined are applicable to a wide range of immunoassay development needs.