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Updated: Jun 4, 2026

Hyperinsulinemic-Euglycemic Clamp in the Conscious Rat

Hyperinsulinemic-Euglycemic Clamp in the Conscious Rat

Published on: February 7, 2011

Hyperinsulinemic-euglycemic clamp in the conscious rat.

Curtis C Hughey¹, Dustin S Hittel, Virginia L Johnsen

¹Department of Biochemistry and Molecular Biology, Faculty of Medicine, University of Calgary. cchughey@ucalgary.ca

Journal of Visualized Experiments : Jove

|February 23, 2011

Summary

This study details a minimally stressful rat model for measuring insulin sensitivity using the hyperinsulinemic-euglycemic clamp technique. This method quantines glucose infusion rates to assess whole-body insulin action in conscious, unrestrained animals.

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Area of Science:

Endocrinology
Metabolic Research
Animal Models

Background:

Type 2 diabetes (T2D) prevalence is increasing globally.
T2D is characterized by insulin resistance or insufficient insulin production, leading to hyperglycemia.
The hyperinsulinemic-euglycemic clamp is the gold standard for assessing insulin sensitivity.

Purpose of the Study:

To present a refined hyperinsulinemic-euglycemic clamp procedure in conscious, unrestrained rats.
To establish a minimally stressful model for accurate insulin sensitivity assessment.
To provide a foundation for further research into tissue-specific glucose uptake and turnover.

Main Methods:

Surgical catheterization of the carotid artery and jugular vein in rats under anesthesia.

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A recovery period of 4-5 days, with daily weight monitoring.

Infusion of insulin and exogenous glucose (D50) in fasted, conscious rats to maintain euglycemia.

Recording glucose levels and infusion rates during stable euglycemic periods.

Main Results:

The described method allows for blood collection with minimal animal stress.
Stable euglycemic conditions were achieved by titrating glucose infusion rates.
Glucose infusion rates directly indicate the index of whole-body insulin sensitivity.

Conclusions:

The chronic catheterized, unrestrained conscious rat model is effective for hyperinsulinemic-euglycemic clamp studies.
This technique provides a reliable measure of whole-body insulin sensitivity.
The method can be adapted for specific research needs and enhanced with radioactive tracers for tissue-specific analysis.