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Updated: Jun 4, 2026

Isolation of Adult Human Dermal Fibroblasts from Abdominal Skin and Generation of Induced Pluripotent Stem Cells Using a Non-Integrating Method
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Published on: January 19, 2020

Methods for making induced pluripotent stem cells: reprogramming à la carte

Federico González1, Stéphanie Boué, Juan Carlos Izpisúa Belmonte

  • 1Center for Regenerative Medicine in Barcelona (CMRB), Dr. Aiguader, 88, 08003, Barcelona, Spain.

Nature Reviews. Genetics
|February 23, 2011
PubMed

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Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore called induced pluripotent stem...
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Chromatin modification alters gene expression; therefore, scientists can add histone-modifying enzymes, histone variants, and chromatin remodeling complexes to somatic cells to aid reprogramming into pluripotent stem (iPS) cells.
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