Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Color-monitored solid-phase multiple peptide synthesis under low-pressure continuous-flow conditions.

V Krchnák1, J Vágner

  • 1Research Institute for Feed Supplements and Veterinary Drugs, Prague, Czechoslovakia.

Peptide Research
|July 1, 1990
PubMed
Summary

Researchers developed rapid solid-phase peptide synthesis for human immunodeficiency virus (HIV) peptides. This method efficiently produces serologically relevant peptides for detecting anti-HIV-1 antibodies.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The medical engineering program of Forschungszentrum Karlsruhe.

Minimally invasive therapy & allied technologies : MITAT : official journal of the Society for Minimally Invasive Therapy·2010
Same author

Convenient and Efficient Synthesis of a Lanthanide-Coordinated, Diethylene Triamine Pentaacetic Acid Labeled Biopolymer as an Assay for the Cholecystokinin B Receptor.

Journal of applied polymer science. Applied polymer symposium·2009
Same author

ROBITOM-robot for biopsy and therapy of the mamma.

Biomedizinische Technik. Biomedical engineering·2002
Same author

New approach for preparation of 2,3,7-trisubstituted 3,4-dihydroisoquinolinone libraries on solid phase.

Molecular diversity·2002
Same author

Robotic system for biopsy and therapy of breast lesions in a high-field whole-body magnetic resonance tomography unit.

Investigative radiology·2000
Same author

Semi-automated high throughput combinatorial solid-phase organic synthesis.

Biotechnology and bioengineering·1999

Area of Science:

  • Chemical Synthesis
  • Biochemistry
  • Immunology

Background:

  • Solid-phase peptide synthesis (SPPS) is crucial for producing peptides.
  • Developing efficient and rapid SPPS methods is essential for research and diagnostics.

Purpose of the Study:

  • To present a rapid solid-phase multiple peptide synthesis method.
  • To demonstrate its application in synthesizing human immunodeficiency virus (HIV) peptides.
  • To evaluate the method's efficiency and the peptides' diagnostic relevance.

Main Methods:

  • Utilized low-pressure continuous-flow reactors for rapid SPPS.
  • Employed concatenated flow reactors with adjustable volume.
  • Monitored acylation completeness using bromophenol blue indicator.

Related Experiment Videos

  • Enhanced coupling efficiency with elevated temperature and ultrasonic bath.
  • Purified crude peptides using mass-overloaded C18 reversed-phase HPLC.

    • Main Results:

    • Successfully synthesized fifty HIV-derived peptides.
    • Demonstrated high efficiency in acylation and coupling reactions.
    • Achieved high recovery of homogeneous peptides post-purification.
    • Synthesized peptides showed serological relevance for anti-HIV-1 antibody detection.

    Conclusions:

    • The described rapid solid-phase peptide synthesis is effective for producing HIV peptides.
    • The method is suitable for generating peptides relevant to anti-HIV-1 antibody detection.
    • This approach offers a valuable tool for diagnostic peptide development.