Neuronal tracing for connectomic studies

Reconstruction of the complete wiring diagram, or connectome, of a neural circuit provides an alternative approach to conventional circuit analysis. One major obstacle of connectomics lies in segmenting and tracing neuronal processes from the vast number of images obtained with optical or electron microscopy. Here I review recent progress in automated tracing algorithms for connectomic reconstruction with fluorescence and electron microscopy, and discuss the challenges to image analysis posed by novel optical imaging techniques.