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Related Experiment Video

Updated: Jun 4, 2026

In vitro Cell Migration and Invasion Assays
09:55

In vitro Cell Migration and Invasion Assays

Published on: June 1, 2014

In vitro invasion assays.

S K Chambers1

  • 1Department of Obstetrics and Gynecology, Yale School of Medicine, New Haven, MA.

Methods in Molecular Medicine
|February 23, 2011
PubMed
Summary
This summary is machine-generated.

Epithelial ovarian cancer invades through protease activity, involving urokinase plasminogen activator (uPA) and matrix metalloproteinases (MMPs). These proteases cooperate to degrade the extracellular matrix, facilitating cancer cell spread and metastasis.

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Area of Science:

  • Oncology
  • Molecular Biology
  • Biochemistry

Background:

  • Epithelial ovarian cancer disseminates via peritoneal implants and lymphatic invasion.
  • Tumor cell invasion is a deregulation of normal physiologic invasion processes.
  • Protease activity and inhibitor balance control normal cell invasion.

Purpose of the Study:

  • To investigate the role of proteases in epithelial ovarian cancer cell invasion.
  • To understand the cooperative mechanisms of different protease classes in extracellular matrix degradation.

Main Methods:

  • Analysis of protease activities, including serine proteases (uPA), matrix metalloproteinases (MMPs), cysteine proteases (cathepsin B), and aspartic proteases (cathepsin D).
  • Examination of protease interactions and their role in extracellular matrix degradation by ovarian cancer cells.

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The Use of Mouse Mammary Tumor Cells in an In Vitro Invasion Assay as a Measure of Oncogenic Cell Behavior
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The Use of Mouse Mammary Tumor Cells in an In Vitro Invasion Assay as a Measure of Oncogenic Cell Behavior

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Last Updated: Jun 4, 2026

In vitro Cell Migration and Invasion Assays
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Published on: June 1, 2014

A Modified In vitro Invasion Assay to Determine the Potential Role of Hormones, Cytokines and/or Growth Factors in Mediating Cancer Cell Invasion
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A Modified In vitro Invasion Assay to Determine the Potential Role of Hormones, Cytokines and/or Growth Factors in Mediating Cancer Cell Invasion

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Main Results:

  • Extracellular matrix-degrading proteases are crucial for tumor invasion and metastasis in epithelial ovarian cancer.
  • Urokinase plasminogen activator (uPA) and matrix metalloproteinases (MMPs) are key players.
  • Cysteine proteases like cathepsin B and aspartic proteases like cathepsin D also contribute to invasion.
  • Proteases cooperate, with uPA activating plasminogen and MMPs, and cathepsin B facilitating uPA action.

Conclusions:

  • Multiple protease classes, including uPA, MMPs, cathepsin B, and cathepsin D, are essential for epithelial ovarian cancer cell invasion.
  • The coordinated action of these proteases optimizes extracellular matrix degradation, promoting cancer spread.