Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

In-situ Hybridization02:31

In-situ Hybridization

In situ hybridization (ISH) is a technique used to detect and localize specific DNA or RNA molecules in cells, tissue, or tissue sections using a labeled probe. The technique was first used in 1969 for the investigation of nucleic acids. It is currently an essential tool in scientific research and clinical settings, especially for diagnostic purposes.
Types of probes and labels
A probe is a complementary strand of DNA or RNA that binds to corresponding nucleotide sequences in a cell. Many...
Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
Karyotyping01:17

Karyotyping

Describing the number and physical features of chromosomes can reveal abnormalities that underlie genetic diseases. This description is facilitated by special staining techniques that produce a particular banding pattern on each chromosome. State-of-the-art techniques make this approach even more powerful, enabling the detection of individual genes that cause disease.A Simple Chromosome Staining Technique Provides Valuable Scientific InsightSome genetic diseases can be detected by looking at...
Southern Blot02:57

Southern Blot

Agarose gel electrophoresis is very useful in separating DNA fragments by size. Running a DNA ladder containing fragments of the known length alongside the sample helps determine the approximate length of the sample DNA fragments. However, additional steps are needed to verify the sequence identity of the sample DNA fragments.
Denatured DNA fragments must be transferred onto a carrier membrane from the gel to make it accessible to a probe - a small ssDNA fragment complementary to the target DNA...
FISH - Fluorescent In-situ Hybridization02:07

FISH - Fluorescent In-situ Hybridization

Fluorescence in situ hybridization, or FISH, was developed in the early 1980s and has quickly become one of the most widely used techniques in cytogenetics. Labeled probes are used to bind complementary DNA or RNA sequences on a chromosome or in a region within a cell. Earlier, the probes could only be obtained by cloning or reverse transcription of a DNA template. Currently, the probe oligonucleotides can be synthesized synthetically. Additionally, with the advancement of optical techniques,...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Duplication 8 [inv dup(8)(p12p23)] with macrocephaly.

Annales de genetique·2002
Same author

Trisomy 4 pter-q12 and monosomy of chromosome 13 pter-q12 in a male with deficiency of all blood lymphocyte populations.

American journal of medical genetics·2001
Same author

Ossifying fibromyxoid tumor of soft parts: report of a case with novel cytogenetic findings.

Cancer genetics and cytogenetics·2001
Same author

Trisomy 5p. A case report and review.

Annales de genetique·2001
Same author

Acute myeloid leukemia associated with hemophagocytic syndrome and t(4;7)(q21;q36).

Cancer genetics and cytogenetics·2000
Same author

Patent ductus arteriosus and microdeletion 22q11 in a patient with Klinefelter syndrome.

Annales de genetique·2000
Same journal

Erratum to: Immunotherapeutic Approach to Cancer with Cutaneous DNA Vaccination.

Methods in molecular medicine·2015
Same journal

Methods for cancer gene therapy using tumor suppressor genes.

Methods in molecular medicine·2014
Same journal

Suppression of the human carcinoma phenotype by an antioncogene ribozyme.

Methods in molecular medicine·2014
Same journal

Methods for the use of stromal cells for therapeutic gene therapy.

Methods in molecular medicine·2014
Same journal

Methods for adenovirus-mediated gene transfer to synovium in vivo.

Methods in molecular medicine·2014
Same journal

Methods for gene transfer to synovium.

Methods in molecular medicine·2014
See all related articles

Related Experiment Video

Updated: Jun 4, 2026

Chromosome Replicating Timing Combined with Fluorescent In situ Hybridization
17:14

Chromosome Replicating Timing Combined with Fluorescent In situ Hybridization

Published on: December 10, 2012

Rapid Identification of Chromosomes Using Primed In Situ Labeling (PRINS).

G V Velagaleti1

  • 1Division of Medical Genetics, Department of Pediatrics,Univeristy of Tennessee, Memphis, TN.

Methods in Molecular Medicine
|February 23, 2011
PubMed
Summary
This summary is machine-generated.

Primed in situ labeling (PRINS) visualizes human chromosome centromeres by targeting variations in alpha satellite DNA sequences. This method allows for chromosome-specific identification, enhancing cytogenetic analysis.

More Related Videos

Simple Method for Fluorescence DNA In Situ Hybridization to Squashed Chromosomes
11:36

Simple Method for Fluorescence DNA In Situ Hybridization to Squashed Chromosomes

Published on: January 6, 2015

Detection of Inter-chromosomal Stable Aberrations by Multiple Fluorescence In Situ Hybridization (mFISH) and Spectral Karyotyping (SKY) in Irradiated Mice
10:14

Detection of Inter-chromosomal Stable Aberrations by Multiple Fluorescence In Situ Hybridization (mFISH) and Spectral Karyotyping (SKY) in Irradiated Mice

Published on: January 11, 2017

Related Experiment Videos

Last Updated: Jun 4, 2026

Chromosome Replicating Timing Combined with Fluorescent In situ Hybridization
17:14

Chromosome Replicating Timing Combined with Fluorescent In situ Hybridization

Published on: December 10, 2012

Simple Method for Fluorescence DNA In Situ Hybridization to Squashed Chromosomes
11:36

Simple Method for Fluorescence DNA In Situ Hybridization to Squashed Chromosomes

Published on: January 6, 2015

Detection of Inter-chromosomal Stable Aberrations by Multiple Fluorescence In Situ Hybridization (mFISH) and Spectral Karyotyping (SKY) in Irradiated Mice
10:14

Detection of Inter-chromosomal Stable Aberrations by Multiple Fluorescence In Situ Hybridization (mFISH) and Spectral Karyotyping (SKY) in Irradiated Mice

Published on: January 11, 2017

Area of Science:

  • Cytogenetics
  • Molecular Biology
  • Genetics

Background:

  • Primed in situ labeling (PRINS) is a technique for visualizing chromosome centromeres.
  • Centromeres are crucial for chromosome segregation during cell division.
  • Human centromeres contain alpha satellite DNA with chromosome-specific variations.

Purpose of the Study:

  • To introduce and explain the principle of Primed in situ labeling (PRINS).
  • To highlight the application of PRINS for chromosome centromere visualization.
  • To demonstrate the potential for chromosome-specific targeting using PRINS.

Main Methods:

  • Utilizing the PRINS technique.
  • Targeting variations within alpha satellite repeat monomers at human centromeres.
  • Leveraging sequence differences for specific probe binding.

Main Results:

  • Successful visualization of human chromosome centromeres.
  • Demonstration of chromosome-specific labeling capabilities.
  • Validation of PRINS as a method for centromere identification.

Conclusions:

  • PRINS is an effective method for centromere visualization.
  • Targeting alpha satellite DNA variations enables chromosome-specific labeling.
  • PRINS offers a valuable tool for cytogenetic studies and genetic analysis.