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Quantitative PCR Detection of c-erbB-2 Gene Amplification
1Department fo Cellular Endocrinology, Ludwig Bolzmann Institute for Experimental Endocrinology, General Hospital of Vienna, Vienna, Austria.
Methods in Molecular Medicine
|February 23, 2011
Abstract:
The association of c-erbB-2 oncogene amplification and prognostic factors was intensively studied in human gynecological carcinomas, especially in mammary carcinoma (1). Positive lymph nodes, estrogen and progesterone receptor negative tumors, and short survival time correlate with c-erbB-2 amplification.
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Real Time RT-PCR
Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...
The real-time quantification of the number of amplified products is...
PCR - Polymerase Chain Reaction
Overview

