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Related Concept Videos

Real Time RT-PCR02:57

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...
PCR - Polymerase Chain Reaction01:32

PCR - Polymerase Chain Reaction

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Quantitative PCR Detection of c-erbB-2 Gene Amplification.

K Dobianer1, M Medl, J Spona

  • 1Department fo Cellular Endocrinology, Ludwig Bolzmann Institute for Experimental Endocrinology, General Hospital of Vienna, Vienna, Austria.

Methods in Molecular Medicine
|February 23, 2011
PubMed
Summary

Amplification of the c-erbB-2 oncogene is linked to poor prognostic factors in gynecological cancers, particularly breast cancer. This includes positive lymph nodes, negative hormone receptors, and shorter survival times.

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Area of Science:

  • Oncology
  • Molecular Biology
  • Gynecologic Oncology

Background:

  • The c-erbB-2 oncogene plays a critical role in cell growth and differentiation.
  • Aberrant expression and amplification of c-erbB-2 are implicated in various human cancers.
  • Understanding the prognostic significance of c-erbB-2 amplification in gynecological carcinomas is crucial for patient management.

Purpose of the Study:

  • To investigate the association between c-erbB-2 oncogene amplification and established prognostic factors in human gynecological carcinomas.
  • To specifically examine this correlation in the context of mammary carcinoma.
  • To determine if c-erbB-2 amplification can serve as a predictive marker for patient outcomes.

Main Methods:

  • Analysis of c-erbB-2 oncogene amplification status in tumor samples.
  • Correlation of amplification status with clinical and pathological prognostic factors, including lymph node status, estrogen receptor (ER) status, and progesterone receptor (PR) status.
  • Evaluation of the relationship between c-erbB-2 amplification and patient survival time.

Main Results:

  • A significant association was observed between c-erbB-2 oncogene amplification and adverse prognostic indicators.
  • Specifically, c-erbB-2 amplification correlated with positive lymph node status.
  • Tumors with c-erbB-2 amplification were more likely to be estrogen and progesterone receptor negative and associated with a shorter survival time.

Conclusions:

  • c-erbB-2 oncogene amplification is a significant molecular marker associated with poor prognostic factors in gynecological carcinomas, especially breast cancer.
  • The findings suggest that c-erbB-2 amplification may predict aggressive tumor behavior and unfavorable outcomes.
  • Further research is warranted to explore therapeutic strategies targeting c-erbB-2 in relevant patient populations.