Related Experiment Video
Updated: Jun 4, 2026
09:06
MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as a Novel Detection and Quantification Method
Published on: October 7, 2025
In Situ Hybridization Detection of TGF-β mRNA.
1Department of Internal Medicine, University of Uppsala Hospital, Uppsala, Sweden.
Methods in Molecular Medicine
|February 23, 2011
Summary
In situ hybridization visualizes specific DNA or RNA sequences within cells or tissues. This molecular technique uses labeled probes to detect and quantify genetic material in biological samples.
Area of Science:
- Molecular Biology
- Genetics
- Cell Biology
Background:
- Immunohistochemistry and molecular hybridization are key techniques for detecting specific biomolecules.
- In situ hybridization (ISH) is a specialized form of molecular hybridization.
Purpose of the Study:
- To describe the principles and applications of in situ hybridization.
- To highlight ISH as a method for detecting nucleic acid sequences in biological specimens.
Main Methods:
- Utilizes single-stranded DNA or RNA probes with complementary sequences.
- Probes are hybridized to target DNA or RNA within tissue sections, cell preparations, or chromosomes.
- Probes are labeled (radioactively or nonisotopically) for detection and quantification.
Main Results:
- Enables precise localization of specific DNA or RNA sequences.
- Allows for the visualization of genetic material within its cellular or tissue context.
- Facilitates the potential quantification of detected nucleic acid sequences.
Conclusions:
- In situ hybridization is a powerful tool for analyzing nucleic acid distribution.
- ISH complements other molecular and histological techniques for biological research.
- The technique is foundational for understanding gene expression and localization.
Related Concept Videos
02:31In-situ Hybridization
In situ hybridization (ISH) is a technique used to detect and localize specific DNA or RNA molecules in cells, tissue, or tissue sections using a labeled probe. The technique was first used in 1969 for the investigation of nucleic acids. It is currently an essential tool in scientific research and clinical settings, especially for diagnostic purposes.
Types of probes and labels
A probe is a complementary strand of DNA or RNA that binds to corresponding nucleotide sequences in a cell. Many...
Types of probes and labels
A probe is a complementary strand of DNA or RNA that binds to corresponding nucleotide sequences in a cell. Many...
01:16TGF - β Signaling Pathway
The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors are of three kinds RI, RII, and RIII. The RI...